Morphogenetic and Cellular Movements that Shape the Mouse Cerebellum: Insights from Genetic Fate Mapping

We used the cerebellum as a model to study the morphogenetic and cellular processes underlying the formation of elaborate brain structures from a simple neural tube, using an inducible genetic fate mapping approach in mouse. We demonstrate how a 90° rotation between embryonic days 9 and 12 converts...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2005-01, Vol.45 (1), p.27-40
Main Authors: Sgaier, Sema K., Millet, Sandrine, Villanueva, Melissa P., Berenshteyn, Frada, Song, Christian, Joyner, Alexandra L.
Format: Article
Language:English
Subjects:
Animals
Body Patterning - genetics
Cell cycle
Cell Differentiation - genetics
Cell Lineage - genetics
Cell Movement - genetics
Cerebellum - cytology
Cerebellum - embryology
Cerebellum - metabolism
Chimera
Female
Gene expression
Gene Expression Regulation, Developmental - genetics
Genetic Markers
Homeodomain Proteins - genetics
Integrases - genetics
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Migration
Morphogenesis - physiology
Nerve Tissue Proteins - genetics
Neurons - cytology
Neurons - metabolism
Promoter Regions, Genetic - genetics
Rodents
Stem Cells - cytology
Stem Cells - metabolism
Studies
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Summary:We used the cerebellum as a model to study the morphogenetic and cellular processes underlying the formation of elaborate brain structures from a simple neural tube, using an inducible genetic fate mapping approach in mouse. We demonstrate how a 90° rotation between embryonic days 9 and 12 converts the rostral-caudal axis of dorsal rhombomere 1 into the medial-lateral axis of the wing-like bilateral cerebellar primordium. With the appropriate use of promoters, we marked specific medial-lateral domains of the cerebellar primordium and derived a positional fate map of the murine cerebellum. We show that the adult medial cerebellum is produced by expansion, rather than fusion, of the thin medial primordium. Furthermore, ventricular-derived cells maintain their original medial-lateral coordinates into the adult, whereas rhombic lip-derived granule cells undergo lateral to medial posterior transverse migrations during foliation. Thus, we show that progressive changes in the axes of the cerebellum underlie its genesis.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2004.12.021