Changes in spectrin organisation in leukaemic and lymphoid cells upon chemotherapy

• Published in: Biochemical pharmacology 2005, Vol.69 (1), p.73-85
• Main Authors: Dubielecka, Patrycja M., Jaźwiec, Bożena, Potoczek, Stanisław, Wróbel, Tomasz, Miłoszewska, Joanna, Haus, Olga, Kuliczkowski, Kazimierz, Sikorski, Aleksander F.
• Format: Article
• Language: English
• Subjects: ALL; Antineoplastic agents; Apoptosis; Biological and medical sciences; Cells, Cultured; Chemotherapy; General aspects; Humans; Lymphocytes - chemistry; Lymphocytes - drug effects; Lymphocytes - metabolism; Lymphoma, Non-Hodgkin - drug therapy; Lymphoma, Non-Hodgkin - metabolism; Medical sciences; nHL; Nonerythroid spectrin (fodrin); Pharmacology. Drug treatments; PKC θ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy; Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism; Spectrin - analysis; Spectrin - metabolism
• ISSN: 0006-2952; 1873-2968
• DOI: 10.1016/j.bcp.2004.08.031

The aim of the present study was to investigate changes in spectrin and protein kinase C θ (PKC θ) organisation in human lymphoid and leukaemic cells undergoing chemotherapeutically induced apoptosis. An analysis of spectrin arrangement in human peripheral lymphoid (non-Hodgkin lymphoma) and leukaemic (acute lymphoblasic leukaemia) cells before and after chemotherapy revealed radical differences in the distribution of this protein. By using immunofluorescent technique, in lymphocytes isolated before chemotherapy, we found spectrin evenly distributed in the cytoplasm and the plasma membrane, while after the therapy changes in spectrin organisation occurred. Moreover, in lymphocytes after chemotherapy, extraction with buffer containing non-ionic detergent (Triton X-100) revealed presence of an insoluble fraction of spectrin. In normal or malignant cells before chemotherapy spectrin was totally soluble, however it should be mentioned that in total cell extracts and supernatants (but not in pellets) apoptotic fragments of spectrin (in addition to intact α and β chains) were also found. In malignant cells after chemotherapy changes in PKC θ organisation, similar to this observed in the case of spectrin, were shown by the immunofluorescence technique. In contrast, no differences in the distribution of other isoforms of protein kinase C: βI and βII, before and after chemotherapy, were found. Apoptotic phosphatidyloserine (PS) externalisation, as well as cell shrinkage, membrane protrusions and blebbing were observed in lymphocytes after chemotherapy and treatment with cytostatics in vitro. The overall results may suggest that spectrin redistribution/aggregation is the phenomenon involved in programmed cell death (PCD) of normal and neoplastic lymphocytes and lymphoblasts, however molecular basis of this phenomenon should be further investigated.