Doxorubicin Uptake and Release from Microgel Thin Films

• Published in: Biomacromolecules 2005-01, Vol.6 (1), p.408-413
• Main Authors: Serpe, Michael J, Yarmey, Kristen A, Nolan, Christine M, Lyon, L. Andrew
• Format: Article
• Language: English
• Subjects: Applied sciences; Biological and medical sciences; Doxorubicin - chemistry; Doxorubicin - pharmacokinetics; Exact sciences and technology; Forms of application and semi-finished materials; Gels - chemical synthesis; Gels - chemistry; General pharmacology; Medical sciences; Membranes, Artificial; Molecular Conformation; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polymer industry, paints, wood; Sheets and films; Spectrophotometry, Ultraviolet; Technology of polymers; Temperature; Time Factors
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm049455x

We report investigations on the thermally regulated uptake and release of the chemotherapeutic drug doxorubicin from microgel thin films. A spin coating, layer-by-layer (scLbL) assembly approach was used to prepare thin films composed of thermoresponsive poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-AAc) microgels by alternatively exposing a 3-aminopropyltrimethoxysilane (APTMS) functionalized glass substrate to polyanionic pNIPAm-AAc microgels and polycationic poly(allylamine hydrochloride) (PAH). Using this method, 10, 20, and 30 microgel layer films were constructed with uniform layer buildup, as confirmed by quartz crystal microgravimetry (QCM). The films were subsequently loaded with doxorubicin by cycling the temperature of the film in an aqueous doxorubicin solution between 25 and 50 °C. Release characteristics were then examined using UV−vis spectroscopy, which revealed temperature-dependent release properties.