3D microenvironment as essential element for osteoinduction by biomaterials

• Published in: Biomaterials 2005-06, Vol.26 (17), p.3565-3575
• Main Authors: Habibovic, Pamela, Yuan, Huipin, van der Valk, Chantal M., Meijer, Gert, van Blitterswijk, Clemens A., de Groot, Klaas
• Format: Article
• Language: English
• Subjects: Animals; Biphasic calcium phosphate (BCP); Bone and Bones - pathology; Bone Substitutes - chemistry; Calcium Phosphates - chemistry; Crystallization - methods; Durapatite - chemistry; Female; Goats; Hydroxyapatite (HA); Implants, Experimental; Macrostructure; Materials Testing; Microstructure; Osseointegration - physiology; Osteogenesis - physiology; Osteoinduction; Porosity; Sintering temperature; Specific surface area; Surface Properties; Temperature; Tissue Engineering - methods
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2004.09.056

In order to unravel the mechanism of osteoinduction by biomaterials, in this study we investigated the influence of the specific surface area on osteoinductive properties of two types of calcium phosphate ceramics. Different surface areas of the ceramics were obtained by varying their sintering temperatures. Hydroxyapatite (HA) ceramic was sintered at 1150 and 1250 °C. Biphasic calcium phosphate (BCP) ceramic, consisting of HA and beta-tricalcium phosphate ( β-TCP), was sintered at 1100, 1150 and 1200 °C. Changes in sintering temperature did not influence the chemistry of the ceramics; HA remained pure after sintering at different temperatures and the weight ratio of HA and β-TCP in the BCP was independent of the temperature as well. Similarly, macroporosity of the ceramics was unaffected by the changes of the sintering temperature. However, microporosity (pore diameter