Hepatitis B Virus Genetic Typing Using Mass-Spectrometry

Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined et...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2009-02, Vol.147 (2), p.220-225
Main Authors: Malakhova, M. V., Ilina, E. N., Govorun, V. M., Shutko, S. A., Dudina, K. R., Znoyko, O. O., Klimova, E. A., Iushchuk, N. D.
Format: Article
Language:English
Subjects:
Base Sequence
Biomedical and Life Sciences
Biomedicine
Cell Biology
Genotype
Hepatitis B
Hepatitis B - virology
Hepatitis B virus
Hepatitis B virus - classification
Hepatitis B virus - genetics
Hepatitis B virus - isolation & purification
Humans
Internal Medicine
Laboratory Medicine
Microbiological Techniques - methods
Molecular Sequence Data
Pathology
Sequence Homology, Nucleic Acid
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Virology
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Summary:Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined etiology and hepatic cirrhosis and allowed to show the prevalence of D genotype (83.3%) in all groups of patients. Other hepatitis B virus genotypes: genotype A (5.9%), genotype C (3.6%), and mixed infection with D and C (7.2%) were also found in patients with chronic hepatitis B and hepatic cirrhosis. All genotypes were found in patients with chronic hepatitis B and hepatic cirrhosis. Chronic hepatitis of combined etiology was noted only in patients with genotype D. Possibility of detection of mixed infection with hepatitis B viruses of various genotypes is a distinct advantage of mini-sequencing approach over direct nucleotide sequence evaluation for hepatitis B virus genetic typing.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-009-0479-1