Nitric oxide, actin reorganization and vacuoles change are involved in PEG 6000-induced stomatal closure in Vicia faba

• Published in: Physiologia plantarum 2009-05, Vol.136 (1), p.45-56
• Main Authors: Huang, Ai-Xia, She, Xiao-Ping, Cao, Bin, Zhang, Bei, Mu, Juan, Zhang, Shao-Jie
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Benzoates - pharmacology; Biological and medical sciences; Cytochalasin B - pharmacology; Fundamental and applied biological sciences. Psychology; Imidazoles - pharmacology; Microscopy, Confocal; NG-Nitroarginine Methyl Ester - pharmacology; Nitric Oxide - biosynthesis; Plant physiology and development; Plant Stomata - drug effects; Plant Stomata - physiology; Plant Transpiration; Polyethylene Glycols - pharmacology; Vacuoles - physiology; Vicia faba - drug effects; Vicia faba - physiology; Water - physiology
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/j.1399-3054.2009.01212.x

Water deficit and the resulting osmotic stress affect stomatal movement. There are two types of signals, hydraulic and chemical signals, involving in the regulation of stomatal behavior responses to osmotic stress. Compared with the chemical signals, little has been known about the hydraulic signals and the corresponding signal transduction network and regulatory mechanisms. Here, using an epidermal-strip bioassay and laser-scanning confocal microscopy, we provide evidence that nitric oxide (NO) generation in Vicia faba guard cells can be induced by hydraulic signals. We used polyethylene glycol (PEG) 600 to simulate hypertonic conditions. This hydraulic signal led to stomatal closure and rapid promotion of NO production in guard cells. The effects were decreased by NO scavenger 2-(4-carboxyphenyl)-4,4,5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) and NO synthase (Enzyme Commission 1.14.13.39) inhibitor NG-nitro- l-Arg-methyl ester ( l-NAME). These results indicate that PEG 6000 induces stomatal closure by promoting NO production. Cytochalasin B (CB) inhibited stomatal closure induced by PEG 6000 but did not prevent the increase of endogenous NO levels, indicating that microfilaments polymerization participate in stomatal closure induced by PEG 6000, and may act downstream of NO signaling. In addition, big vacuoles split into many small vacuoles were observed in response to PEG 6000 and sodium nitroprusside (SNP) treatment, and CB inhibited these changes of vacuoles, the stomatal closure was also been inhibited. Collectively, these results suggest that the stomatal closure induced by PEG 6000 may be intimately associated with NO levels, reorganization of actin filaments and the changes of vacuoles, showing a crude outline of guard-cells signaling process in response to hydraulic signals.