Protection of mitochondrial system by Hippophae rhamnoides L. against radiation-induced oxidative damage in mice

The whole extract of the fresh berries of Hippophae rhamnoides L. (RH−3), which has been reported to provide protection to whole mice, various tissues, cells and cell organelles against lethal irradiation, was further investigated for its effects on mitochondria isolated from mouse liver. Superoxide...

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Bibliographic Details
Published in:Journal of pharmacy and pharmacology 2005-01, Vol.57 (1), p.135-143
Main Authors: Goel, Harish Chandra, Gupta, Damodar, Gupta, Shobha, Garg, A. P., Bala, Madhu
Format: Article
Language:English
Subjects:
Animals
Electron Transport Complex I - metabolism
Fruit - chemistry
Glutathione - metabolism
Hippophae - chemistry
Lipid Peroxidation - drug effects
Male
Membrane Potentials - drug effects
Mice
Mitochondria, Liver - drug effects
Mitochondria, Liver - enzymology
Mitochondria, Liver - radiation effects
Oxidation-Reduction
Oxidative Stress - drug effects
Oxidative Stress - radiation effects
Plant Extracts
Proteins - chemistry
Proteins - metabolism
Radiation-Protective Agents
Succinate Cytochrome c Oxidoreductase - metabolism
Superoxides - metabolism
Whole-Body Irradiation
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Summary:The whole extract of the fresh berries of Hippophae rhamnoides L. (RH−3), which has been reported to provide protection to whole mice, various tissues, cells and cell organelles against lethal irradiation, was further investigated for its effects on mitochondria isolated from mouse liver. Superoxide anion, reduced (GSH) and oxidized glutathione (GSSG) levels, NADH‐ubiquinone oxidoreductase (complex I), NADH‐cytochrome c oxidoreductase (complex I/II), succinate‐cytochrome c oxidoreductase (complex II/III), mitochondrial membrane potential (MMP), lipid peroxidation (LPx) and protein oxidation (PO) were determined for RH‐3‐mediated radioprotective manifestation. Pre‐irradiation treatment of mice with RH‐3 (30 mg kg−1, i.p.; single dose; −30 min) significantly inhibited the radiation‐induced increase in superoxide anions, GSSG, thiobarbituric acid reactive substances (TBARS), complex I, complex I/III activity and MMP maximally at 4 h (P < 0.05). This treatment inhibited the oxidation of proteins (P < 0.05) at all the time periods studied here. This study suggests that pre‐irradiation treatment of mice with RH‐3 protects the functional integrity of mitochondria from radiation‐induced oxidative stress.
ISSN:0022-3573
2042-7158
DOI:10.1211/0022357055218