Specific expression of pfkfb4 gene in spermatogonia germ cells and analysis of its 5′-flanking region

• Published in: FEBS letters 2005-01, Vol.579 (2), p.357-362
• Main Authors: Gómez, Marta, Manzano, Anna, Navarro-Sabaté, Àurea, Duran, Joan, Obach, Mercè, Perales, Jose C., Bartrons, Ramon
• Format: Article
• Language: English
• Subjects: 5' Flanking Region - genetics; 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase; Animals; Base Sequence; Cell Line; DHT; dihydrotestosterone; Exons - genetics; Gene Expression; Gene Expression Regulation - drug effects; Genes, Reporter; Germ Cells - metabolism; glucocorticoid-response element; Glycolysis; GRE; HRE; Humans; hypoxia-response element; Introns - genetics; L-PFK-2; liver PFK-2; Luciferases - genetics; Male; Male germ cell; Mice; Molecular Sequence Data; PDB; PFK-2; phorbol 12,13-dibutyrate; Phosphofructokinase-2 - biosynthesis; Phosphofructokinase-2 - genetics; Promoter; Promoter Regions, Genetic - genetics; Rats; RNA, Messenger - analysis; RNA, Messenger - metabolism; Sequence Deletion - genetics; Spermatogonia - cytology; Spermatogonia - metabolism; Testis; Testis - chemistry; Testis - metabolism; testis PFK-2; Tissue Distribution; tPFK-2; ubiquitous PFK-2; uPFK-2
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2004.11.096

The results presented demonstrate the expression of pfkfb4 gene in adult testis and in a mouse spermatogonia germ cell line (GC-1spg). The genomic organization of the human pfkfb4 gene shows the existence of 14 exons and 13 introns, spanning 45 kb. A detailed analysis of the 5′-flanking region by transient transfection assays with different 5′-deletion promoter constructs in GC-1spg and mouse sertoli cells (TM-4), allows us to define the minimal promoter unit, containing several GC-rich and ETF sequences along the first −141 nucleotides involved in basal expression. This gene is activated by serum and chemical hypoxia (CoCl2 treatment) whereas β-estradiol decreases its expression.