New azo dyes as colored isoelectric point markers for isoelectric focusing in acidic pH region

Newly prepared azo compounds and several commercially available indicators were investigated for their applicability as colored isoelectric point (pI) markers for isoelectric focusing (IEF) in the acidic range below pH 5. The majority of compounds described here can serve as primary standards since...

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Bibliographic Details
Published in:Electrophoresis 2005-01, Vol.26 (1), p.53-59
Main Authors: Stastná, Miroslava, Trávnícek, Martin, Slais, Karel
Format: Article
Language:English
Subjects:
Ampholytic dyes
Azo Compounds - chemical synthesis
Azo Compounds - chemistry
Coloring Agents - chemical synthesis
Coloring Agents - chemistry
Hydrogen-Ion Concentration
Isoelectric Focusing
Isoelectric Point
p/ markers
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Summary:Newly prepared azo compounds and several commercially available indicators were investigated for their applicability as colored isoelectric point (pI) markers for isoelectric focusing (IEF) in the acidic range below pH 5. The majority of compounds described here can serve as primary standards since their pI values were determined by UV–VIS spectrophotometry independently IEF and direct measurement with a pH electrode. Subjected to gel IEF they show narrow and well‐observable zones of different colors. Finally, our work resulted in suggestion of a color ladder composed of pI markers covering the pH range from 1.5 to 4.7.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200406088