Impaired T- and B-cell development in Tcl1-deficient mice

TCL1, the overexpression of which may result in T-cell leukemia, is normally expressed in early embryonic tissues, the ovary, and lymphoid lineage cells. Our analysis of mouse B-lineage cells indicates that Tcl1 expression is initiated in pro-B cells and persists in splenic marginal zone and follicu...

Full description

Saved in:
Bibliographic Details
Published in:Blood 2005-02, Vol.105 (3), p.1288-1294
Main Authors: Kang, Sang-Moo, Narducci, Maria Grazia, Lazzeri, Cristina, Mongiovì, Adriana M., Caprini, Elisabetta, Bresin, Antonella, Martelli, Fabio, Rothstein, Jay, Croce, Carlo Maria, Cooper, Max D., Russo, Giandomenico
Format: Article
Language:English
Subjects:
Animals
Antibody Formation
B-Lymphocytes - immunology
Biological and medical sciences
Cell Division
Cell Survival
DNA Primers
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Mice
Mice, Knockout
Multigene Family
Polymerase Chain Reaction
Proto-Oncogene Proteins - deficiency
Proto-Oncogene Proteins - genetics
T-Lymphocytes - immunology
Transcription, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:TCL1, the overexpression of which may result in T-cell leukemia, is normally expressed in early embryonic tissues, the ovary, and lymphoid lineage cells. Our analysis of mouse B-lineage cells indicates that Tcl1 expression is initiated in pro-B cells and persists in splenic marginal zone and follicular B cells. T-lineage Tcl1 expression begins in thymocyte progenitors, continues in CD4+CD8+ thymocytes, and is extinguished in mature T cells. In Tcl1-deficient mice, we found B lymphopoiesis to be compromised at the pre-B cell stage and T-cell lymphopoiesis to be impaired at the CD4+CD8+ thymocyte stage. A corresponding increase was observed in thymocyte susceptibility to anti-CD3ϵ–induced apoptosis. Reduced numbers of splenic follicular and germinal center B cells were accompanied by impaired production of immunoglobulin G1 (IgG1) and IgG2b antibodies in response to a T-dependent antigen. The marginal zone B cells and T-cell–independent antibody responses were also diminished in Tcl1-/- mice. This analysis indicates a significant role for Tcl1, a coactivator of Akt signaling, in normal T- and B-cell development and function.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood-2004-04-1453