Rapid Method for Simultaneous Quantitative Determination of Four Major Essential Oil Components from Oregano (Oreganum sp.) and Thyme (Thymus sp.) Using FT-Raman Spectroscopy

A simple and rapid method for the quantitative determination of four major components found in oregano and thyme essential oils is presented. The method correlates the Raman peak intensity in the spectral region from 1800 to 600 cm-1 and the concentration percentage of each particular constituent in...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2005-01, Vol.53 (2), p.202-206
Main Authors: Siatis, Nikolaos G, Kimbaris, Athanasios C, Pappas, Christos S, Tarantilis, Petros A, Daferera, Dimitra J, Polissiou, Moschos G
Format: Article
Language:English
Subjects:
Aroma and flavouring agent industries
Biological and medical sciences
carvacrol
essential oils
food analysis
Food industries
Fourier transform Raman spectroscopy
Fundamental and applied biological sciences. Psychology
gamma-terpinene
Oils, Volatile - analysis
oregano
Origanum
Origanum - chemistry
p-cymene
Raman spectroscopy
rapid methods
Reproducibility of Results
Sensitivity and Specificity
Software
Spectrum Analysis, Raman - methods
thyme
thymol
Thymus
Thymus Plant - chemistry
Thymus vulgaris
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Summary:A simple and rapid method for the quantitative determination of four major components found in oregano and thyme essential oils is presented. The method correlates the Raman peak intensity in the spectral region from 1800 to 600 cm-1 and the concentration percentage of each particular constituent in the sample. To achieve accurate quantification results and avoid the risk of overlapping peaks of unknown Raman-active substances in natural essential oils, the peaks must be analyzed. For this purpose, PEAKSOLVE software (Ver. 1.0.5) was used. Unknown samples were measured with the FT-Raman method, and the results were compared to those of the gas chromatographic (GC) analysis. The comparison was made at a confidence level of 99%, and the two methods scored equally in terms of repeatability and accuracy even at the edge of the method specifications. The new method can provide accurate results in very short times once the setup is complete and could be utilized in areas where vast amounts of samples must be analyzed. Keywords: FT-Raman; oregano; thyme; essential oil; quantitative; deconvolution
ISSN:0021-8561
1520-5118
DOI:10.1021/jf048930f