Rapid detection of methicillin resistance in teicoplanin-resistant coagulase-negative staphylococci by a penicillin-binding protein 2′ latex agglutination method

Thirty-five clinical isolates of coagulase-negative staphylococci with decreased glycopeptide sensitivity were examined by a penicillin-binding protein (PBP2′) latex agglutination (LA) test and were compared to the detection of the mecA gene by PCR, and oxacillin susceptibility determined minimum in...

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Bibliographic Details
Published in:Journal of microbiological methods 2005-03, Vol.60 (3), p.413-416
Main Authors: Knausz, Márta, Ghidán, Ágoston, Grossato, Anna, Rozgonyi, Ferenc
Format: Article
Language:English
Subjects:
Anti-Infective Agents - pharmacology
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Carbon-Oxygen Ligases - chemistry
Carbon-Oxygen Ligases - genetics
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
Humans
Latex Fixation Tests - methods
Methicillin Resistance - physiology
Microbial Sensitivity Tests
Microbiology
Miscellaneous
Penicillin binding protein
Penicillin-Binding Proteins - chemistry
Polymerase Chain Reaction
Staphylococcal Infections - microbiology
Staphylococcus
Staphylococcus epidermidis - drug effects
Staphylococcus epidermidis - genetics
Staphylococcus epidermidis - metabolism
Staphylococcus haemolyticus - drug effects
Staphylococcus haemolyticus - genetics
Staphylococcus haemolyticus - metabolism
Teicoplanin - pharmacology
Teicoplanin resistance
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Summary:Thirty-five clinical isolates of coagulase-negative staphylococci with decreased glycopeptide sensitivity were examined by a penicillin-binding protein (PBP2′) latex agglutination (LA) test and were compared to the detection of the mecA gene by PCR, and oxacillin susceptibility determined minimum inhibitory concentrations. The latex test demonstrated high sensitivity and specificity for detecting methicillin resistance in coagulase-negative staphylococci after PBP2′ induction with oxacillin.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2004.09.019