Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1

The erythroblast transformation specific (ETS) transcription factor GA-binding protein (Gabp) is widely expressed and acts on a diverse range of target genes, including nuclear-encoded mitochondrial proteins and neuromuscular-specific genes. The GABPalpha subunit contains an ETS DNA binding domain a...

Full description

Saved in:
Bibliographic Details
Published in:Gene 2005-01, Vol.344, p.79-92
Main Authors: O'Leary, Debra A, Koleski, Daniela, Kola, Ismail, Hertzog, Paul J, Ristevski, Sika
Format: Article
Language:English
Subjects:
3T3 Cells
Alternative Splicing - genetics
Animals
Binding Sites - genetics
DNA, Complementary - chemistry
DNA, Complementary - genetics
DNA-Binding Proteins - genetics
Embryo, Mammalian - metabolism
Female
GA-Binding Protein Transcription Factor
Gene Expression Profiling
Gene Expression Regulation, Developmental
In Situ Hybridization
Luciferases - genetics
Luciferases - metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Molecular Sequence Data
Promoter Regions, Genetic - genetics
Protein Subunits - genetics
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Analysis, DNA
Transcription Factors - genetics
Transcription, Genetic - genetics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The erythroblast transformation specific (ETS) transcription factor GA-binding protein (Gabp) is widely expressed and acts on a diverse range of target genes, including nuclear-encoded mitochondrial proteins and neuromuscular-specific genes. The GABPalpha subunit contains an ETS DNA binding domain and the beta subunit contains a nuclear localization signal (NLS) and transactivation domain. Here, we show coincident expression of Gabpalpha and beta1 throughout mouse embryogenesis, consistent with the gene products functioning in a complex. We have also identified 2 alternatively spliced, tissue-specific exons 1 (5' untranslated regions) of mouse Gabpalpha and 4 alternative 3' polyadenylation signals that, in combination, result in 12 transcripts for Gabpalpha. These alternative transcripts are suggested to have altered stability, subcellular localization and/or translation efficiency. Further, we identified nine differentially expressed splice variants of mouse Gabpbeta1 that encode beta protein forms lacking functional domains, suggesting a dominant negative function. Together, alternative transcripts of Gabpalpha and beta1 provide a mechanism for tissue-specific regulation of Gabp activity.
ISSN:0378-1119