Direct Observation of Anion-Mediated Translocation of Fluorescent Oligoarginine Carriers into and across Bulk Liquid and Anionic Bilayer Membranes

The recent hypothesis that counteranion-mediated dynamic inversion of charge and solubility might contribute to diverse functions of oligoarginines in biomembranes was tested with two fluorescently labelled oligomers, FL-R₈, one of the most active cell-penetrating peptides, and its longer version, F...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2005-01, Vol.6 (1), p.114-122
Main Authors: Sakai, Naomi, Takeuchi, Toshihide, Futaki, Shiroh, Matile, Stefan
Format: Article
Language:English
Subjects:
anions
Antiporters - chemistry
Antiporters - physiology
Arginine - chemistry
bioorganic chemistry
Fluorescent Dyes - chemistry
ion pairs
lipid bilayers
Lipid Bilayers - chemistry
oligoarginines
Oligopeptides - chemistry
Protein Transport
Solubility
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Summary:The recent hypothesis that counteranion-mediated dynamic inversion of charge and solubility might contribute to diverse functions of oligoarginines in biomembranes was tested with two fluorescently labelled oligomers, FL-R₈, one of the most active cell-penetrating peptides, and its longer version, FL-R₁₆. We report evidence for counteranion-mediated phase transfer from water into bulk chloroform and anionic lipid-bilayer membranes as well as reverse-phase transfer from bulk chloroform and across intact lipid-bilayer membranes into water. The differences found between FL-R₈ and FL-R₁₆ with regard to location in the bilayer and reverse-phase transfer from bulk and lipid-bilayer membranes into water implied that the reported results may be relevant for biological function.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200400256