Pro- and anti-inflammatory cytokines gene polymorphisms and Helicobacter pylori infection: interactions influence outcome

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2005-02, Vol.29 (4), p.141-152
• Main Authors: Zambon, Carlo-F., Basso, Daniela, Navaglia, Filippo, Belluco, Claudio, Falda, Alessandra, Fogar, Paola, Greco, Eliana, Gallo, Nicoletta, Rugge, Massimo, Di Mario, Francesco, Plebani, Mario
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Cytokines - genetics; Duodenal Ulcer - etiology; Duodenal Ulcer - genetics; Female; Gastric cancer; Gastritis - etiology; Gastritis - genetics; Gene Frequency; Genotype; Helicobacter Infections - complications; Helicobacter Infections - diagnosis; Helicobacter Infections - genetics; Helicobacter pylori; Helicobacter pylori - genetics; Helicobacter pylori - pathogenicity; Humans; Interleukin-1 - genetics; Interleukin-1 alpha; Interleukin-10; Interleukin-10 - genetics; Intestinal Diseases - etiology; Intestinal Diseases - genetics; Italy; Male; Metaplasia - etiology; Metaplasia - genetics; Middle Aged; Polymorphism, Genetic - genetics; Stomach Neoplasms - etiology; Stomach Neoplasms - genetics; Stomach Ulcer - etiology; Stomach Ulcer - genetics; Tumor necrosis factor alpha; Tumor Necrosis Factor-alpha - genetics; Virulence - genetics
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2004.10.013

The aim of this study was to evaluate whether there was any correlation between Helicobacter pylori-associated diseases and (1) H. pylori virulence genes or (2) IL-1B, IL-1RN, IFN-G, TNF-A, IL-10 genetic polymorphisms. Patients with non-cardia gastric cancer (NCGC, n = 129) or benign gastroduodenal diseases ( n = 792) were studied. IL-1RN intron 2 VNTR polymorphism (PCR), IL-1B −31 C/T (RFLP), the SNPs of IFN-G (+874 A/T), TNF-A (−1031 C/T, −857 C/T, −376 A/G, −308 A/G, −238 A/G), IL-10 (−1082 A/G, −819 C/T, −592 A/C) (Taqman chemistry) were studied. cagA, s1 and m1 vacA, were PCR amplified. Duodenal ulcer was more frequent in TNF-A −857 TT and in IL-1RN 1,2 subjects. TNF-A −857 TT genotype was also correlated with gastric ulcer. IL-10 −819 TT genotype was associated with intestinal metaplasia and NCGC. Antral inflammation was associated with TNF-A −1031 TT, while corpus activity with IL-10 −819 CC. H. pylori infection was associated with TNF-A −308 AG genotype, while IFN-G +874 AA genotype was associated with cagA. In conclusion, among host genetic factors contributing to H. pylori disease outcome, IFN-G +874 AA genotype favors cagA positive infections, TNF-A −857 TT duodenal ulcer while IL-10 −819 TT intestinal metaplasia and NCGC.