In vitro evaluation of the biocompatibility of contaminated implant surfaces treated with an Er : YAG laser and an air powder system

: Titanium platelets with a sand‐blasted and acid‐etched surface were coated with bovine serum albumin and incubated with a suspension of Porphyromonas gingivalis (ATCC 33277). Four groups with a total of 48 specimens were formed. Laser irradiation of the specimens (n=12) was performed on a computer...

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Published in:Clinical oral implants research 2005-02, Vol.16 (1), p.36-43
Main Authors: Kreisler, Matthias, Kohnen, Wolfgang, Christoffers, Ann-Babett, Götz, Hermann, Jansen, Bernd, Duschner, Heinz, D'Hoedt, Bernd
Format: Article
Language:English
Subjects:
Adolescent
Air Abrasion, Dental
air powder cleaning
biocompatibility
Biocompatible Materials
Cell Proliferation
Cells, Cultured
Decontamination - instrumentation
Decontamination - methods
Dental Implants - microbiology
Dentistry
Erbium
Er : YAG laser
Fibroblasts - cytology
Fluorescence
Gingiva - cytology
Humans
implant surface
Lasers
periimplantitis
Porphyromonas gingivalis - radiation effects
Powders
Surface Properties
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Summary:: Titanium platelets with a sand‐blasted and acid‐etched surface were coated with bovine serum albumin and incubated with a suspension of Porphyromonas gingivalis (ATCC 33277). Four groups with a total of 48 specimens were formed. Laser irradiation of the specimens (n=12) was performed on a computer‐controlled XY translation stage at pulse energy 60 mJ and frequency 10 pps. Twelve specimens were treated with an air powder system. After the respective treatment, human gingival fibroblasts were incubated on the specimens. The proliferation rate was determined by means of fluorescence activity of a redox indicator (Alamar Blue® Assay) which is reduced by metabolic activity related to cellular growth. Proliferation was determined up to 72 h. Contaminated and non‐treated as well as sterile specimens served as positive and negative controls. Proliferation activity was significantly (Mann–Whitney U‐test, P
ISSN:0905-7161
1600-0501
DOI:10.1111/j.1600-0501.2004.01056.x