Determination of cocaine and benzoylecgonine by direct injection of human urine into a column-switching liquid chromatography system with diode-array detection

• Published in: Journal of pharmaceutical and biomedical analysis 2005-02, Vol.37 (1), p.115-120
• Main Authors: Brunetto, M.R., Cayama, Y. Delgado, García, L. Gutiérrez, Gallignani, M., Obando, M.A.
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Benzoylecgonine; Biological and medical sciences; Chromatography, Liquid - methods; Cocaine; Cocaine - analogs & derivatives; Cocaine - analysis; Cocaine - urine; Column switching; Flow Injection Analysis - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC; Humans; Medical sciences; Pharmacology. Drug treatments; Sample preparation; Solid-phase extraction; Substance Abuse Detection - methods; Urine samples
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2004.09.048

A method for the determination of cocaine (COC) and benzoylecgonine (BZE) in human urine using a column-switching liquid chromatography system is reported. A homemade precolumn (20 mm × 4.6 mm i.d.) dry-packed with Alltech ODS-C18 (35–750 μm) was employed as an extraction precolumn in order to extract and concentrate the COC and BZE from the human urine sample. The analytes were continuously transferred to the analytical column (Spherisorb-C8, 250 mm × 4.6 mm i.d.; dp = 5 μm) by means of the switching arrangement in the backflush mode. Detection was carried out at 235 nm in a UV-diode array detector. The validation of the method revealed analytes quantitative recoveries (96–102%) at three concentrations in the range from 0.25 to 4.00 and from 0.5 to 12.0 μg/mL for COC and BZE, respectively. These values demonstrate the excellent extraction efficiency of the precolumn. The detection limits for COC and BZE at a signal-to-noise ratio of 3 were 0.08 and 0.15 μg/mL when a sample volume of 50 μL was injected. The overlap of sample preparation, analysis and recondition of the precolumn increases the sample throughput to four samples per hour. The proposed method has been applied to the determination of COC and BZE in human urine samples from 73 suspecting drug addicts. Urine concentrations of 1.0–118.10 μg of BZE/mL and 0.1–41.0 μg of COC/mL were found.