Loading…
Determination of cocaine and benzoylecgonine by direct injection of human urine into a column-switching liquid chromatography system with diode-array detection
A method for the determination of cocaine (COC) and benzoylecgonine (BZE) in human urine using a column-switching liquid chromatography system is reported. A homemade precolumn (20 mm × 4.6 mm i.d.) dry-packed with Alltech ODS-C18 (35–750 μm) was employed as an extraction precolumn in order to extra...
Saved in:
| Published in: | Journal of pharmaceutical and biomedical analysis 2005-02, Vol.37 (1), p.115-120 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c384t-20bca8eb6f822c51c1def475b2b9942ab4108764468cec8d964339efac94353f3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c384t-20bca8eb6f822c51c1def475b2b9942ab4108764468cec8d964339efac94353f3 |
| container_end_page | 120 |
| container_issue | 1 |
| container_start_page | 115 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 37 |
| creator | Brunetto, M.R. Cayama, Y. Delgado García, L. Gutiérrez Gallignani, M. Obando, M.A. |
| description | A method for the determination of cocaine (COC) and benzoylecgonine (BZE) in human urine using a column-switching liquid chromatography system is reported. A homemade precolumn (20
mm
×
4.6
mm i.d.) dry-packed with Alltech ODS-C18 (35–750
μm) was employed as an extraction precolumn in order to extract and concentrate the COC and BZE from the human urine sample. The analytes were continuously transferred to the analytical column (Spherisorb-C8, 250
mm
×
4.6
mm i.d.; dp
=
5
μm) by means of the switching arrangement in the backflush mode. Detection was carried out at 235
nm in a UV-diode array detector. The validation of the method revealed analytes quantitative recoveries (96–102%) at three concentrations in the range from 0.25 to 4.00 and from 0.5 to 12.0
μg/mL for COC and BZE, respectively. These values demonstrate the excellent extraction efficiency of the precolumn. The detection limits for COC and BZE at a signal-to-noise ratio of 3 were 0.08 and 0.15
μg/mL when a sample volume of 50
μL was injected. The overlap of sample preparation, analysis and recondition of the precolumn increases the sample throughput to four samples per hour. The proposed method has been applied to the determination of COC and BZE in human urine samples from 73 suspecting drug addicts. Urine concentrations of 1.0–118.10
μg of BZE/mL and 0.1–41.0
μg of COC/mL were found. |
| doi_str_mv | 10.1016/j.jpba.2004.09.048 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67372627</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708504004856</els_id><sourcerecordid>67372627</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-20bca8eb6f822c51c1def475b2b9942ab4108764468cec8d964339efac94353f3</originalsourceid><addsrcrecordid>eNp9kU2L1TAUhoM4ONfRP-BCsnF2rflq2oIbmfELBmaj4C6k6eltSpvcSdqR-mf8q6bewuxcHQjP--YkD0JvKMkpofL9kA-nRueMEJGTOieieoYOtCp5xqT4-RwdSMlpVpKquEQvYxwIIQWtxQt0SQspRVmQA_pzCzOEyTo9W--w77DxRlsHWLsWN-B--3UEc_RuO2tW3NoAZsbWDWnskX6ZtMNL2BDrZo91ahmXyWXxl51Nb90Rj_ZhsS02ffCTnv0x6FO_4rjGGSacqD41-xYyHYJOt6St_tW_QhedHiO83ucV-vH50_ebr9nd_ZdvNx_vMsMrMWeMNEZX0MiuYswU1NAWuvTChjV1LZhuBCVVKYWQlQFTtbUUnNfQaVMLXvCOX6Hrc-8p-IcF4qwmGw2Mo3bgl6hkyUsmWZlAdgZN8DEG6NQp2EmHVVGiNi1qUJsWtWlRpFZJSwq93duXZoL2KbJ7SMC7HdDR6LEL2hkbnzgpZMHLOnEfzhykv3i0EFQ0FpyBsxbVevu_Pf4Csw2wDQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67372627</pqid></control><display><type>article</type><title>Determination of cocaine and benzoylecgonine by direct injection of human urine into a column-switching liquid chromatography system with diode-array detection</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Brunetto, M.R. ; Cayama, Y. Delgado ; García, L. Gutiérrez ; Gallignani, M. ; Obando, M.A.</creator><creatorcontrib>Brunetto, M.R. ; Cayama, Y. Delgado ; García, L. Gutiérrez ; Gallignani, M. ; Obando, M.A.</creatorcontrib><description>A method for the determination of cocaine (COC) and benzoylecgonine (BZE) in human urine using a column-switching liquid chromatography system is reported. A homemade precolumn (20
mm
×
4.6
mm i.d.) dry-packed with Alltech ODS-C18 (35–750
μm) was employed as an extraction precolumn in order to extract and concentrate the COC and BZE from the human urine sample. The analytes were continuously transferred to the analytical column (Spherisorb-C8, 250
mm
×
4.6
mm i.d.; dp
=
5
μm) by means of the switching arrangement in the backflush mode. Detection was carried out at 235
nm in a UV-diode array detector. The validation of the method revealed analytes quantitative recoveries (96–102%) at three concentrations in the range from 0.25 to 4.00 and from 0.5 to 12.0
μg/mL for COC and BZE, respectively. These values demonstrate the excellent extraction efficiency of the precolumn. The detection limits for COC and BZE at a signal-to-noise ratio of 3 were 0.08 and 0.15
μg/mL when a sample volume of 50
μL was injected. The overlap of sample preparation, analysis and recondition of the precolumn increases the sample throughput to four samples per hour. The proposed method has been applied to the determination of COC and BZE in human urine samples from 73 suspecting drug addicts. Urine concentrations of 1.0–118.10
μg of BZE/mL and 0.1–41.0
μg of COC/mL were found.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2004.09.048</identifier><identifier>PMID: 15664750</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Benzoylecgonine ; Biological and medical sciences ; Chromatography, Liquid - methods ; Cocaine ; Cocaine - analogs & derivatives ; Cocaine - analysis ; Cocaine - urine ; Column switching ; Flow Injection Analysis - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; HPLC ; Humans ; Medical sciences ; Pharmacology. Drug treatments ; Sample preparation ; Solid-phase extraction ; Substance Abuse Detection - methods ; Urine samples</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2005-02, Vol.37 (1), p.115-120</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-20bca8eb6f822c51c1def475b2b9942ab4108764468cec8d964339efac94353f3</citedby><cites>FETCH-LOGICAL-c384t-20bca8eb6f822c51c1def475b2b9942ab4108764468cec8d964339efac94353f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16465379$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15664750$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brunetto, M.R.</creatorcontrib><creatorcontrib>Cayama, Y. Delgado</creatorcontrib><creatorcontrib>García, L. Gutiérrez</creatorcontrib><creatorcontrib>Gallignani, M.</creatorcontrib><creatorcontrib>Obando, M.A.</creatorcontrib><title>Determination of cocaine and benzoylecgonine by direct injection of human urine into a column-switching liquid chromatography system with diode-array detection</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A method for the determination of cocaine (COC) and benzoylecgonine (BZE) in human urine using a column-switching liquid chromatography system is reported. A homemade precolumn (20
mm
×
4.6
mm i.d.) dry-packed with Alltech ODS-C18 (35–750
μm) was employed as an extraction precolumn in order to extract and concentrate the COC and BZE from the human urine sample. The analytes were continuously transferred to the analytical column (Spherisorb-C8, 250
mm
×
4.6
mm i.d.; dp
=
5
μm) by means of the switching arrangement in the backflush mode. Detection was carried out at 235
nm in a UV-diode array detector. The validation of the method revealed analytes quantitative recoveries (96–102%) at three concentrations in the range from 0.25 to 4.00 and from 0.5 to 12.0
μg/mL for COC and BZE, respectively. These values demonstrate the excellent extraction efficiency of the precolumn. The detection limits for COC and BZE at a signal-to-noise ratio of 3 were 0.08 and 0.15
μg/mL when a sample volume of 50
μL was injected. The overlap of sample preparation, analysis and recondition of the precolumn increases the sample throughput to four samples per hour. The proposed method has been applied to the determination of COC and BZE in human urine samples from 73 suspecting drug addicts. Urine concentrations of 1.0–118.10
μg of BZE/mL and 0.1–41.0
μg of COC/mL were found.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Benzoylecgonine</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>Cocaine</subject><subject>Cocaine - analogs & derivatives</subject><subject>Cocaine - analysis</subject><subject>Cocaine - urine</subject><subject>Column switching</subject><subject>Flow Injection Analysis - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>HPLC</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Sample preparation</subject><subject>Solid-phase extraction</subject><subject>Substance Abuse Detection - methods</subject><subject>Urine samples</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp9kU2L1TAUhoM4ONfRP-BCsnF2rflq2oIbmfELBmaj4C6k6eltSpvcSdqR-mf8q6bewuxcHQjP--YkD0JvKMkpofL9kA-nRueMEJGTOieieoYOtCp5xqT4-RwdSMlpVpKquEQvYxwIIQWtxQt0SQspRVmQA_pzCzOEyTo9W--w77DxRlsHWLsWN-B--3UEc_RuO2tW3NoAZsbWDWnskX6ZtMNL2BDrZo91ahmXyWXxl51Nb90Rj_ZhsS02ffCTnv0x6FO_4rjGGSacqD41-xYyHYJOt6St_tW_QhedHiO83ucV-vH50_ebr9nd_ZdvNx_vMsMrMWeMNEZX0MiuYswU1NAWuvTChjV1LZhuBCVVKYWQlQFTtbUUnNfQaVMLXvCOX6Hrc-8p-IcF4qwmGw2Mo3bgl6hkyUsmWZlAdgZN8DEG6NQp2EmHVVGiNi1qUJsWtWlRpFZJSwq93duXZoL2KbJ7SMC7HdDR6LEL2hkbnzgpZMHLOnEfzhykv3i0EFQ0FpyBsxbVevu_Pf4Csw2wDQ</recordid><startdate>20050207</startdate><enddate>20050207</enddate><creator>Brunetto, M.R.</creator><creator>Cayama, Y. Delgado</creator><creator>García, L. Gutiérrez</creator><creator>Gallignani, M.</creator><creator>Obando, M.A.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050207</creationdate><title>Determination of cocaine and benzoylecgonine by direct injection of human urine into a column-switching liquid chromatography system with diode-array detection</title><author>Brunetto, M.R. ; Cayama, Y. Delgado ; García, L. Gutiérrez ; Gallignani, M. ; Obando, M.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-20bca8eb6f822c51c1def475b2b9942ab4108764468cec8d964339efac94353f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Benzoylecgonine</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>Cocaine</topic><topic>Cocaine - analogs & derivatives</topic><topic>Cocaine - analysis</topic><topic>Cocaine - urine</topic><topic>Column switching</topic><topic>Flow Injection Analysis - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>HPLC</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Sample preparation</topic><topic>Solid-phase extraction</topic><topic>Substance Abuse Detection - methods</topic><topic>Urine samples</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brunetto, M.R.</creatorcontrib><creatorcontrib>Cayama, Y. Delgado</creatorcontrib><creatorcontrib>García, L. Gutiérrez</creatorcontrib><creatorcontrib>Gallignani, M.</creatorcontrib><creatorcontrib>Obando, M.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brunetto, M.R.</au><au>Cayama, Y. Delgado</au><au>García, L. Gutiérrez</au><au>Gallignani, M.</au><au>Obando, M.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of cocaine and benzoylecgonine by direct injection of human urine into a column-switching liquid chromatography system with diode-array detection</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2005-02-07</date><risdate>2005</risdate><volume>37</volume><issue>1</issue><spage>115</spage><epage>120</epage><pages>115-120</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A method for the determination of cocaine (COC) and benzoylecgonine (BZE) in human urine using a column-switching liquid chromatography system is reported. A homemade precolumn (20
mm
×
4.6
mm i.d.) dry-packed with Alltech ODS-C18 (35–750
μm) was employed as an extraction precolumn in order to extract and concentrate the COC and BZE from the human urine sample. The analytes were continuously transferred to the analytical column (Spherisorb-C8, 250
mm
×
4.6
mm i.d.; dp
=
5
μm) by means of the switching arrangement in the backflush mode. Detection was carried out at 235
nm in a UV-diode array detector. The validation of the method revealed analytes quantitative recoveries (96–102%) at three concentrations in the range from 0.25 to 4.00 and from 0.5 to 12.0
μg/mL for COC and BZE, respectively. These values demonstrate the excellent extraction efficiency of the precolumn. The detection limits for COC and BZE at a signal-to-noise ratio of 3 were 0.08 and 0.15
μg/mL when a sample volume of 50
μL was injected. The overlap of sample preparation, analysis and recondition of the precolumn increases the sample throughput to four samples per hour. The proposed method has been applied to the determination of COC and BZE in human urine samples from 73 suspecting drug addicts. Urine concentrations of 1.0–118.10
μg of BZE/mL and 0.1–41.0
μg of COC/mL were found.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15664750</pmid><doi>10.1016/j.jpba.2004.09.048</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2005-02, Vol.37 (1), p.115-120 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67372627 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Analysis Analytical, structural and metabolic biochemistry Benzoylecgonine Biological and medical sciences Chromatography, Liquid - methods Cocaine Cocaine - analogs & derivatives Cocaine - analysis Cocaine - urine Column switching Flow Injection Analysis - methods Fundamental and applied biological sciences. Psychology General pharmacology HPLC Humans Medical sciences Pharmacology. Drug treatments Sample preparation Solid-phase extraction Substance Abuse Detection - methods Urine samples |
| title | Determination of cocaine and benzoylecgonine by direct injection of human urine into a column-switching liquid chromatography system with diode-array detection |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T11%3A13%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20cocaine%20and%20benzoylecgonine%20by%20direct%20injection%20of%20human%20urine%20into%20a%20column-switching%20liquid%20chromatography%20system%20with%20diode-array%20detection&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=Brunetto,%20M.R.&rft.date=2005-02-07&rft.volume=37&rft.issue=1&rft.spage=115&rft.epage=120&rft.pages=115-120&rft.issn=0731-7085&rft.eissn=1873-264X&rft.coden=JPBADA&rft_id=info:doi/10.1016/j.jpba.2004.09.048&rft_dat=%3Cproquest_cross%3E67372627%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c384t-20bca8eb6f822c51c1def475b2b9942ab4108764468cec8d964339efac94353f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=67372627&rft_id=info:pmid/15664750&rfr_iscdi=true |