Isolation of recombinant antibodies (scFvs) to grapevine virus B

A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia...

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Bibliographic Details
Published in:Journal of virological methods 2005-03, Vol.124 (1), p.191-195
Main Authors: Saldarelli, P., Keller, H., Dell’Orco, M., Schots, A., Elicio, V., Minafra, A.
Format: Article
Language:English
Subjects:
Antibodies, Viral - immunology
Antibodies, Viral - isolation & purification
Biological and medical sciences
ELISA
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Fundamental and applied biological sciences. Psychology
Grapevine virus B
Immunoglobulin Fragments - immunology
Immunoglobulin Fragments - isolation & purification
Microbiology
Peptide Library
Phage display
Plant Viruses - immunology
Recombinant antibodies
Recombinant Fusion Proteins - immunology
Recombinant Fusion Proteins - isolation & purification
scFv
Techniques used in virology
Virology
Vitaceae
Vitis - virology
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Summary:A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2004.11.021