Evidence of heterogeneity in the antibody response against the platelet antigen 3a; recognition of an 11-mer peptide carrying the HPA-3a polymorphic determinant

Background  The immune processes involved in the development of alloantibodies against the human platelet antigens in alloimmune disorders remain unclear. Antibody recognition of the platelet antigens on their respective platelet glycoproteins has been shown to be dependent on glycoprotein conformat...

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Bibliographic Details
Published in:Vox sanguinis 2009-04, Vol.96 (3), p.252-255
Main Authors: De Leon, E. J., Yuan, F-F., Pearson, H., Marquis, C. P., Mahler, S. M.
Format: Article
Language:English
Subjects:
alloantibodies
Antibody Formation - genetics
Antigens
Antigens, Human Platelet - chemistry
Antigens, Human Platelet - genetics
Antigens, Human Platelet - immunology
Autoimmune Diseases - blood
Autoimmune Diseases - immunology
Blood products
Blood transfusions
Epitopes - chemistry
Epitopes - genetics
Epitopes - immunology
Female
Glycoproteins
human platelet antigens
Humans
Isoantibodies - blood
Isoantibodies - chemistry
Isoantibodies - immunology
Male
Peptides - chemistry
Peptides - genetics
Peptides - immunology
Polymorphism, Genetic - immunology
thrombocytopenia
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Summary:Background  The immune processes involved in the development of alloantibodies against the human platelet antigens in alloimmune disorders remain unclear. Antibody recognition of the platelet antigens on their respective platelet glycoproteins has been shown to be dependent on glycoprotein conformation. Furthermore, the post‐translational modification of glycoproteins adds complexity to the alloantigenic determinants. Methods  Nine anti‐HPA‐3a sera along with several control sera were tested for reactivity to an 11‐mer peptide straddling the HPA‐3a/b polymorphism. Sera found to specifically recognize the 3a peptide were further assessed by platelet pre‐exposure and immunoblotting. Results  Three of the nine antisera were found to specifically recognize an 11‐mer synthetic 3a peptide by ELISA. Further analysis of all anti‐HPA‐3a sera by Western blot showed that only those reactive to the 3a peptide were able to bind both reduced and non‐reduced GPIIb. Conclusion  The results presented in this study provide the first known evidence for the identification of an antibody population capable of recognizing a linear and non‐glycosylated form of the HPA‐3a epitope.
ISSN:0042-9007
1423-0410
DOI:10.1111/j.1423-0410.2008.01146.x