Analytical quantification of low-density lipoprotein using europium tetracycline indicator

Low‐density lipoprotein (LDL) is known as ‘bad’ cholesterol. If too much LDL circulates in the blood it can be retained in the walls of the arteries, causing atherosclerosis. In this paper we showed an alternative method to quantify LDL using the europium tetracycline (EuTc) indicator. The optical p...

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Bibliographic Details
Published in:Luminescence (Chichester, England) England), 2009-05, Vol.24 (3), p.189-193
Main Authors: De Oliveira Silva, Flávia Rodrigues, Monteiro, Andrea Moreira, Neto, Antônio M. Figueiredo, Gidlund, Magnus, Junior, Nilson Dias Vieira, Courrol, Lilia Coronato
Format: Article
Language:English
Subjects:
europium
Humans
Indicators and Reagents - analysis
Indicators and Reagents - chemistry
Lipoproteins, LDL - blood
low-density lipoprotein
Luminescence
optical spectroscopy
Organometallic Compounds - analysis
Organometallic Compounds - chemistry
Reference Values
Sensitivity and Specificity
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Tetracyclines - analysis
Tetracyclines - chemistry
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Summary:Low‐density lipoprotein (LDL) is known as ‘bad’ cholesterol. If too much LDL circulates in the blood it can be retained in the walls of the arteries, causing atherosclerosis. In this paper we showed an alternative method to quantify LDL using the europium tetracycline (EuTc) indicator. The optical properties of the EuTc complex were investigated in aqueous solutions containing LDL. An enhancement was observed of the europium luminescence in the solutions with LDL compared those without the lipoprotein. A method to quantify the amount of LDL in a sample, based on EuTc enhanced luminescence, is proposed. The enhancement mechanism is also discussed. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:1522-7235
1522-7243
DOI:10.1002/bio.1094