Evaluation of the Lipid Content in Bovine Oocytes and Embryos with Nile Red: a Practical Approach

Contents In this study, the fluorescent lipid dye Nile Red, was used to demonstrate that the lipid content of immature bovine oocytes is correlated with the morphological appearance of the ooplasm. Oocytes with a uniform dark cytoplasm contained significantly more intracellular lipids in lipid dropl...

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Bibliographic Details
Published in:Reproduction in domestic animals 2005-02, Vol.40 (1), p.76-78
Main Authors: Leroy, JLMR, Genicot, G, Donnay, I, Van Soom, A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Culture Media
Dyes
Embryos
Female
Fertilization in Vitro - methods
Fertilization in Vitro - veterinary
Fundamental and applied biological sciences. Psychology
Lipid Metabolism
Lipids
Mammalian reproduction. General aspects
Morula - metabolism
Oocytes - metabolism
Oxazines
Pregnancy
Vertebrates: reproduction
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Summary:Contents In this study, the fluorescent lipid dye Nile Red, was used to demonstrate that the lipid content of immature bovine oocytes is correlated with the morphological appearance of the ooplasm. Oocytes with a uniform dark cytoplasm contained significantly more intracellular lipids in lipid droplets compared with oocytes with a granulated or pale cytoplasm (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2004.00556.x