Gene expression of ecdysteroid-regulated gene E74 of the honeybee in ovary and brain

To facilitate studies of hormonal control in the honeybee (Apis mellifera L.), a cDNA for a honeybee homologue of the ecdysteroid-regulated gene E74 (AmE74) was isolated and its expression was analysed. Northern blot analysis indicated strong expression in the adult queen abdomen, and no significant...

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Bibliographic Details
Published in:Insect molecular biology 2005, Vol.14 (1), p.9-15
Main Authors: Paul, R.K, Takeuchi, H, Matsuo, Y, Kubo, T
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Animals
Apidae
Apis mellifera
Base Sequence
Bees - genetics
Bees - metabolism
Blotting, Northern
brain
Brain - metabolism
Brain - physiology
caste
Cloning, Molecular
complementary DNA
ecdysteroid
ecdysteroids
Ecdysteroids - genetics
Ecdysteroids - physiology
Expressed Sequence Tags
Female
gene expression regulation
Gene Expression Regulation - physiology
genes
honeybee
hormonal regulation
In Situ Hybridization
Insect Proteins - biosynthesis
Insect Proteins - genetics
messenger RNA
Molecular Sequence Data
mushroom bodies
mushroom body
nucleotide sequences
Open Reading Frames
ovaries
ovary
Ovary - metabolism
Ovary - physiology
queen honey bees
Random Amplified Polymorphic DNA Technique
Sequence Alignment
worker honey bees
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Summary:To facilitate studies of hormonal control in the honeybee (Apis mellifera L.), a cDNA for a honeybee homologue of the ecdysteroid-regulated gene E74 (AmE74) was isolated and its expression was analysed. Northern blot analysis indicated strong expression in the adult queen abdomen, and no significant expression in the adult drone and worker abdomens. In situ hybridization demonstrated that this gene was expressed selectively in the ovary and gut in the queen abdomen. Furthermore, this gene was also expressed selectively in subsets of mushroom body interneurones in the brain of the adult worker bees. These findings suggest that AmE74 is involved in neural function as well as in reproduction in adult honeybees.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2004.00524.x