Sequence Analysis of the Hypervariable Regions of the 56 kDa Immunodominant Protein Genes of Orientia tsutsugamushi Strains in Malaysia

The DNA sequences encompassing two hypervariable regions, VD II and III of the 56 kDa immunodominant protein gene of 21 Malaysian strains of Orientia tsutsugamushi were determined. Two strains demonstrated a 100% DNA homology with the Gilliam prototype strain, and one with TH1817 strain and TA678 st...

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Bibliographic Details
Published in:Microbiology and immunology 2005-01, Vol.49 (1), p.67-71
Main Authors: Tay, Sun Tee, Rohani, Yasin Mohd, Ho, Tze Ming, Shamala, Devi
Format: Article
Language:English
Subjects:
56 KDa immunodominant protein gene
Antigens, Bacterial - genetics
Bacterial Proteins - genetics
DNA, Bacterial - genetics
Genes, Bacterial
Malaysia
Molecular Sequence Data
Orientia tsutsugamushi
Orientia tsutsugamushi - classification
Orientia tsutsugamushi - genetics
Orientia tsutsugamushi - immunology
Phylogeny
Polymorphism, Genetic
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
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Summary:The DNA sequences encompassing two hypervariable regions, VD II and III of the 56 kDa immunodominant protein gene of 21 Malaysian strains of Orientia tsutsugamushi were determined. Two strains demonstrated a 100% DNA homology with the Gilliam prototype strain, and one with TH1817 strain and TA678 strain respectively. High percentages of DNA similarity (95–99%) were observed with Karp (4 strains), Gilliam (2 strains), TH1817 (4 strains), TC586 (3 strains) and TA763 (1 strain). The remaining strains demonstrated the highest DNA similarity with TA763 (1 strain, 89%), TA678 (1 strain, 86%) and TA686 (1 strain, 87%). Our study provides additional evidence on the existence and the genetic heterogeneity of TA strains of the Southeast Asia and their closely related strains in Malaysia.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2005.tb03641.x