Light induction of the carotenoid biosynthesis pathway in Blakeslea trispora

A gene of Blakeslea trispora has been cloned by heterologous hybridization with the Mucor circinelloides crgA gene, a repressor of light-inducible carotenogenesis. This gene is the ortholog of the M. circinelloides crgA, since it was able to restore the wild-type phenotype of a null crgA mutant of M...

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Bibliographic Details
Published in:Fungal genetics and biology 2005-02, Vol.42 (2), p.141-153
Main Authors: Quiles-Rosillo, María D., Ruiz-Vázquez, Rosa M., Torres-Martínez, Santiago, Garre, Victoriano
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
beta-carotene
biosynthesis
Blakeslea trispora
Carotene biosynthesis
carotenes
Carotenoids - biosynthesis
Carotenoids - genetics
Choanephora
Complementation
crgA gene
Filamentous fungi
Fungal Proteins - genetics
gene expression
Gene Expression - radiation effects
Gene Expression Regulation, Fungal - genetics
Genes, Fungal - genetics
Genetic Complementation Test
Heterologous expression
Light
Light regulation
messenger RNA
Molecular Sequence Data
Mucor circinelloides
Mucorales - genetics
Mucorales - physiology
nucleotide sequences
Photoadaptation
polypeptides
Protein Structure, Tertiary
regulator genes
Repetitive Sequences, Nucleic Acid - genetics
Sequence Alignment
Sequence Homology, Amino Acid
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Summary:A gene of Blakeslea trispora has been cloned by heterologous hybridization with the Mucor circinelloides crgA gene, a repressor of light-inducible carotenogenesis. This gene is the ortholog of the M. circinelloides crgA, since it was able to restore the wild-type phenotype of a null crgA mutant of M. circinelloides. The expression of B. trispora crgA gene is light-induced and photoadapted, as occurs for M. circinelloides crgA. Light induction and photoadaptation of B. trispora crgA was also observed in M. circinelloides, which suggests that the mechanisms involved in light regulation are basically conserved between these filamentous fungi. Conservation of the regulatory pathway that controls carotene biosynthesis was supported by the light-induced and photoadapted expression of all structural carotenogenic genes of B. trispora. Consequently, the β-carotene content of dark grown mycelia of B. trispora increased upon illumination with white light.
ISSN:1087-1845
1096-0937
DOI:10.1016/j.fgb.2004.10.008