The 30-kDa band from Salmonella typhimurium: IgM, IgA and IgG antibody response in patients with ankylosing spondylitis

• Published in: Rheumatology (Oxford, England) England), 2009-07, Vol.48 (7), p.748-754
• Main Authors: Zambrano-Zaragoza, Jose F., de Jesus Durán-Avelar, Ma, Rodríguez-Ocampo, Angelica N., García-Latorre, Ethel, Burgos-Vargas, Ruben, Dominguez-Lopez, Maria-L., Pena-Virgen, Salvador, Vibanco-Pérez, Norberto
• Format: Article
• Language: English
• Subjects: Adult; Animals; Ankylosing spondylitis; Antibody Formation; Antibody levels; Antigens, Bacterial - isolation & purification; Antigens, Bacterial - pharmacology; Arthritis, Rheumatoid - immunology; Autoimmunity; Bacterial Proteins - isolation & purification; Bacterial Proteins - pharmacology; Biological and medical sciences; Blotting, Western - methods; Case-Control Studies; Chi-Square Distribution; Diseases of the osteoarticular system; Diseases of the spine; Electrophoresis, Polyacrylamide Gel - methods; Enzyme-Linked Immunosorbent Assay; Female; Genetic Predisposition to Disease; HLA-B27 Antigen - immunology; Humans; Immunoglobulin A - immunology; Immunoglobulin G - immunology; Immunoglobulin M - immunology; Immunoglobulins - analysis; Inflammatory joint diseases; Male; Medical sciences; Middle Aged; Salmonella; Salmonella typhimurium; Salmonella typhimurium - immunology; Spondylitis, Ankylosing - genetics; Spondylitis, Ankylosing - immunology; Young Adult
• ISSN: 1462-0324; 1462-0332
• DOI: 10.1093/rheumatology/kep113

Objective. To determine the association of Salmonella typhimurium antigens with AS by analysing the IgA, IgG and IgM antibody response to the crude lysate and the 30-kDa band from this micro-organism. Methods. Sera from 28 AS patients, 28 HLA-B27+ healthy relatives, 28 unrelated healthy subjects and 14 RA patients were included. Salmonella typhimurium proteins were electrophoretically separated and blotted onto nitrocellulose sheets for immunodetection with sera from AS patients and unrelated healthy subjects. The electroeluted 30-kDa band (p30) and a crude lysat (StCL) from S. typhimurium were used as antigen to evaluate the IgM, IgA and IgG (total and subclasses) antibody levels by ELISA. An inhibition assay was carried out to confirm the specificity of IgG response to the p30. Results. Twenty out of 28 AS patients (71.4%) and 4 out of 28 unrelated healthy subjects (14.3%) recognized a 30-kDa band from S. typhimurium with IgG antibodies. Six out of 28 AS patients (21.4%) and 4 out of 28 unrelated healthy subjects (14.3%) detected it with IgA antibodies. Recognition of p30 and StCL by both IgA and IgG antibodies was higher in AS patients than in control groups (P = 0.003,