A Stopped Flow System with Hydrodynamic Injection for Red Blood Cells Osmotic Fragility Test: Possibility for Automatic Screening of Beta-thalassemia Trait

Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline soluti...

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Bibliographic Details
Published in:Analytical Sciences 2009/06/10, Vol.25(6), pp.819-824
Main Authors: KHONYOUNG, Supada, HARTWELL, Supaporn KRADTAP, JAKMUNEE, Jaroon, LAPANANTNOPPAKHUN, Somchai, SANGUANSERMSRI, Torpong, GRUDPAN, Kate
Format: Article
Language:English
Subjects:
Analytical Chemistry
beta-Thalassemia - diagnosis
beta-Thalassemia - pathology
Chemistry
Erythrocytes - metabolism
Flow Injection Analysis - instrumentation
Flow Injection Analysis - methods
Humans
Osmotic Fragility
Sensitivity and Specificity
Spectrophotometry
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Summary:Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.25.819