Method for the Determination of Vitamin K Homologues in Human Plasma Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry

We report here the development of a precise and sensitive method for the determination of vitamin K homologues including phylloquinone (PK), menaquinone-4 (MK-4), and menaquinone-7 (MK-7) in human plasma using HPLC-tandem mass-mass spectrometry with atmospheric pressure chemical ionization (LC-APCI-...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2005-02, Vol.77 (3), p.757-763
Main Authors: Suhara, Yoshitomo, Kamao, Maya, Tsugawa, Naoko, Okano, Toshio
Format: Article
Language:English
Subjects:
Analytical chemistry
Calibration
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Chromatography, High Pressure Liquid - methods
Exact sciences and technology
Fluids
Humans
Mass spectrometry
Mass Spectrometry - methods
Osteoporosis - diagnosis
Other chromatographic methods
Oxygen Isotopes
Plasma
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrometric and optical methods
Spectrometry, Fluorescence - methods
Vitamin K - analogs & derivatives
Vitamin K - blood
Vitamin K 1 - blood
Vitamin K 2 - analogs & derivatives
Vitamin K 2 - blood
Vitamins
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Summary:We report here the development of a precise and sensitive method for the determination of vitamin K homologues including phylloquinone (PK), menaquinone-4 (MK-4), and menaquinone-7 (MK-7) in human plasma using HPLC-tandem mass-mass spectrometry with atmospheric pressure chemical ionization (LC-APCI-MS/MS). The method involves the use of stable isotope 18O-labeled internal standard compounds, which were synthesized in our laboratory, and the selection of a precursor and product ion with a MS/MS multiple reaction monitoring method. The average intraassay and interassay variation values for PK, MK-4, and MK-7 were
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0489667