On increasing protein-crystallization throughput for X-ray diffraction studies

Two recent developments, a novel screening/optimization strategy that considerably reduces the number of trials required to produce diffraction‐size crystals and a simple modification that doubles the screening capacity of the Douglas Instruments ORYX 1‐6 protein‐crystallization robot, have been imp...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2005-02, Vol.61 (2), p.123-129
Main Authors: Shah, Ashit K., Liu, Zhi-Jie, Stewart, Patrick D., Schubot, Florian D., Rose, John P., Newton, M. Gary, Wang, Bi-Cheng
Format: Article
Language:English
Subjects:
combination screen
Crystallization
high-throughput
Protein Conformation
Proteins - chemistry
robots
X-Ray Diffraction
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Summary:Two recent developments, a novel screening/optimization strategy that considerably reduces the number of trials required to produce diffraction‐size crystals and a simple modification that doubles the screening capacity of the Douglas Instruments ORYX 1‐6 protein‐crystallization robot, have been implemented into a structural genomics project. The new two‐step screening/optimization strategy yields diffraction‐quality crystals directly from the screening process, reducing the need for further optimization. The ORYX modification involves the addition of extensions to the sample‐ and oil‐delivery arms and software modifications that allow two plates to be set up simultaneously.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444904027064