Patterns of myocardial histogenesis as revealed by mouse chimeras

In order to study the pattern of clonal myocyte distribution during mammalian heart development, we have exploited embryo aggregation chimeras using, as cellular markers, an enhanced jellyfish green fluorescent protein (eGFP) transgene and a desmin-promoter-driven, nuclear-localized β-galactosidase...

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Bibliographic Details
Published in:Developmental biology 2005-02, Vol.278 (2), p.336-346
Main Authors: Eberhard, Daniel, Jockusch, Harald
Format: Article
Language:English
Subjects:
3D reconstruction
Animals
Blastocyst - cytology
Blastocyst - physiology
Body Patterning
Cell labeling
Cell marking
Chimera - genetics
Clone
eGFP
Embryo Transfer
Female
Fetal Development
Gene Expression Regulation, Developmental
Green Fluorescent Proteins - genetics
Heart - embryology
Heart - growth & development
Heart development
Image Processing, Computer-Assisted
Mice
Mice, Transgenic
Morula - cytology
Mouse chimera
Myocardium
nLacZ
Patches
Pseudopregnancy
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Summary:In order to study the pattern of clonal myocyte distribution during mammalian heart development, we have exploited embryo aggregation chimeras using, as cellular markers, an enhanced jellyfish green fluorescent protein (eGFP) transgene and a desmin-promoter-driven, nuclear-localized β-galactosidase (nlacZ) knock-in. In neonatal, weanling, and adult chimeric atria and ventricles, irregularly formed patches of various sizes rather than highly dispersed cardiomyocytes were observed. Most of the smaller patches and single cardiomyocytes were found in spatial neighborhood of large patches. This indicated largely coherent clonal growth during myocardial histogenesis combined with tangential displacement or active migration of myocytes. The patterns of ventricular walls were simpler than those of the septum and the atria. In the adult heart, large myocardial volumes devoid of eGFP-positive cardiomyocytes indicated a lack of secondary immigration of blood-borne stem cells into the myocardium. The patterns of oligoclonal expansions revealed in this work might be helpful in detecting and analyzing cell-lineage-based pathological processes in the heart.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2004.11.015