JAK and STAT proteins are expressed and activated by IFN-γ in rat pancreatic acinar cells

• Published in: Journal of cellular physiology 2005-04, Vol.203 (1), p.209-216
• Main Authors: Gallmeier, E., Schäfer, C., Moubarak, P., Tietz, A., Plössl, I., Huss, R., Göke, B., Wagner, A.C.C.
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - pharmacology; Cell Nucleus - metabolism; Cholecystokinin - pharmacology; Cytosol - metabolism; DNA-Binding Proteins - metabolism; Enzyme Inhibitors - pharmacology; Epidermal Growth Factor - pharmacology; Interferon-gamma - pharmacology; Interleukin-6 - pharmacology; Janus Kinase 1; Janus Kinase 2; Male; Milk Proteins - metabolism; Pancreas, Exocrine - cytology; Pancreas, Exocrine - drug effects; Pancreas, Exocrine - metabolism; Phosphorylation - drug effects; Protein-Tyrosine Kinases - metabolism; Proto-Oncogene Proteins - metabolism; Rats; Rats, Sprague-Dawley; STAT1 Transcription Factor; STAT2 Transcription Factor; STAT3 Transcription Factor; STAT5 Transcription Factor; STAT6 Transcription Factor; Trans-Activators - metabolism; Tumor Necrosis Factor-alpha - pharmacology; TYK2 Kinase; Tyrphostins - pharmacology
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.20216

The development of acute pancreatitis (AP) is triggered by acinar events, but the subsequent extra‐acinar events, particularly a distinct immune response, appear to determine its severity. Cytokines modulate this immune response and are derived not only from immunocytes but also from pancreatic acinar cells. We studied whether pancreatic acinar cells were also capable of responding to cytokines. The JAK/STAT‐pathway represents the main effector for many cytokines. Therefore, expression and regulation of JAK and STAT proteins were investigated in rat pancreatic acinar cells. Western blotting showed expression of JAK1, JAK2, Tyk2, and STAT1, STAT2, STAT3, STAT5, STAT6. In addition, STAT1 was reversibly tyrosine‐phosphorylated upon the procedure of acinar cell isolation. In contrast, STAT3‐phosphorylation occurred spontaneously after pancreas removal and was not reversible within 8 h. STAT1 phosphorylation was also observed upon treatment with IFN‐γ but not upon EGF, TNF‐α or IL‐6, and inhibited by the JAK2‐inihibitor AG‐490. Immunohistochemistry revealed cytoplasmic expression of unphosphorylated STAT1 in untreated acinar cells and nuclear translocation of phosphorylated STAT1 following IFN‐γ‐treatment. Interestingly, although CCK leads to the activation of multiple stress pathways in pancreatic acinar cells, we found no influence of CCK on phosphorylation of STAT1, STAT3, or STAT5 in the pancreas. In conclusion, our data provide further evidence that pancreatic acinar cells are able to interact with immune cells. Besides stimulating immune cells via cytokine secretion, acinar cells are in turn capable of responding to IFN‐γ via JAK2 and STAT1 which may have an impact on the development of AP. © 2004 Wiley‐Liss, Inc.