Molecular cloning of chicken hepatic histidase and the regulation of histidase mRNA expression by dietary protein

Chicken hepatic histidase activity varies with dietary protein consumption, but the mechanisms responsible for this alteration in activity are unclear. In the present research, the complete coding sequence and deduced amino acid sequence for chicken histidase was determined from clones isolated from...

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Bibliographic Details
Published in:The Journal of nutritional biochemistry 2005-02, Vol.16 (2), p.114-120
Main Authors: Chendrimada, Thimmaiah P., Davis, Adam J.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blotting, Northern
Chicken
Chickens - genetics
Cloning, Molecular
Dietary protein
Dietary Proteins - pharmacology
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic - drug effects
Histidase
Histidine - administration & dosage
Histidine Ammonia-Lyase - chemistry
Histidine Ammonia-Lyase - genetics
Humans
Liver - enzymology
Mice
Molecular Sequence Data
Rats
RNA, Messenger - analysis
Sequence Homology
Temporal change
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Chicken hepatic histidase activity varies with dietary protein consumption, but the mechanisms responsible for this alteration in activity are unclear. In the present research, the complete coding sequence and deduced amino acid sequence for chicken histidase was determined from clones isolated from a chicken liver cDNA library. The deduced amino acid sequence of chicken histidase has greater than 85% identity with the amino acid sequences of rat, mouse, and human histidase. In a series of four experiments, broiler chicks were allowed free access for 1.5, 3, 6, or 24 h to a low (13 g/100 g diet), basal (22 g/100 g diet) and high (40 g/100 g diet) protein diet. In the final experiment 5, chicks were allowed free access for 24 h to the basal, high protein diet or the basal diet supplemented with three different levels of l-histidine (0.22 g/100 g diet, 0.43 g/100 g diet or 0.86 g/100 g diet). There were no differences in the expression of the mRNA for histidase at 1.5 h, but at 3 h, histidase mRNA expression was significantly ( P
ISSN:0955-2863
1873-4847
DOI:10.1016/j.jnutbio.2004.10.008