Acid hydrolysis and quantitative determination of total hexosamines of an exopolysaccharide produced by Citrobacter sp

During the hydrolysis of an exopolysaccharide (EPS) produced by Citrobacter sp., the maximum liberation of hexosamine was obtained with 6 M HCl at 115 degrees C in an autoclave for 1 h. The glycosidic bond energy and degree of acetylation of the hexosamine in EPS were approximately 77 kJ mol(-1) and...

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Bibliographic Details
Published in:Biotechnology letters 2005, Vol.27 (1), p.13-18
Main Authors: JANG, Jin-Ho, HUI CHING HIA, IKE, Michihiko, INOUE, Chiyo, FUJITA, Masanori, YOSHIDA, Toshiomi
Format: Article
Language:English
Subjects:
Acetylation
Acids - chemistry
Autoclaves
Biological and medical sciences
Biotechnology
Citrobacter
Citrobacter - metabolism
Culture
Destruction
EPS
Fundamental and applied biological sciences. Psychology
Hexosamines
Hexosamines - analysis
Hydrolysis
Polysaccharides - analysis
Polysaccharides - biosynthesis
Polysaccharides - metabolism
Thermodynamics
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Summary:During the hydrolysis of an exopolysaccharide (EPS) produced by Citrobacter sp., the maximum liberation of hexosamine was obtained with 6 M HCl at 115 degrees C in an autoclave for 1 h. The glycosidic bond energy and degree of acetylation of the hexosamine in EPS were approximately 77 kJ mol(-1) and 61%, respectively. Thermal destruction of the hexosamines and the effect of salt on the hexosamine determination could be minimized under the optimized hydrolytic conditions. Using a modified Elson-Morgan method, maximum total hexosamine concentration was determined to be 3.2 g l(-1) (29% of crude EPS) after 96 h of fed-batch culture.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-004-6305-y