Cost efficient and effective gene transfer into the human natural killer cell line, NK92

Introducing genes into cells is a crucial step in many fields of basic research, as well as for the development of new drugs and therapies. Many cell types are resistant to normal methods of gene delivery, such as lipid based transfection and electroporation. Delivery to resistant cell lines can be...

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Bibliographic Details
Published in:Journal of immunological methods 2005, Vol.296 (1), p.31-36
Main Authors: Grund, Eric M., Muise-Helmericks, Robin C.
Format: Article
Language:English
Subjects:
Antigens, CD - analysis
Antigens, CD - genetics
Antigens, CD - physiology
B7-2 Antigen
Biological and medical sciences
Buffers
Cell Line
Electroporation
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Killer Cells, Natural - cytology
Killer Cells, Natural - immunology
Membrane Glycoproteins - analysis
Membrane Glycoproteins - genetics
Membrane Glycoproteins - physiology
Molecular immunology
NK cells
RNA, Small Interfering - genetics
RNAi
Techniques
Transfection - economics
Transfection - methods
Transient expression
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Summary:Introducing genes into cells is a crucial step in many fields of basic research, as well as for the development of new drugs and therapies. Many cell types are resistant to normal methods of gene delivery, such as lipid based transfection and electroporation. Delivery to resistant cell lines can be costly or inefficient. Natural killer (NK) cells are highly resistant to transfection. We have developed a novel method to deliver exogenous genes in the NK cell line, NK92. Using a combination of electroporation and a defined buffer, we were able to obtain an electroporation efficiency of 40% in NK92 cells. Using RNAi, we show significant reduction of an endogenous protein (ETS1) using this optimized buffer and electroporation conditions. Taken together, the results show a functional and cost effective method for the expression of exogenous genes in NK cells.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2004.10.008