Use of magnetic beads to extract fungal DNA

Summary Authors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum,...

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Bibliographic Details
Published in:Mycoses 2005-01, Vol.48 (1), p.3-7
Main Authors: Faggi, E., Pini, G., Campisi, E.
Format: Article
Language:English
Subjects:
Arthrodermataceae - classification
Arthrodermataceae - genetics
Candida albicans
Candida albicans - classification
Candida albicans - genetics
Chloroform
Cryptococcus neoformans
Cryptococcus neoformans - classification
Cryptococcus neoformans - genetics
DNA extraction
DNA, Fungal - analysis
DNA, Fungal - isolation & purification
Epidermophyton floccosum
fungal DNA
Humans
magnetic beads
Magnetics
Microsporum canis
Mycological Typing Techniques
Phenol
Polymerase Chain Reaction - methods
Polystyrenes
Trichophyton rubrum
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Summary:Summary Authors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum, Trichophyton rubrum, T. interdigitale, T. ajelloi, Epidermophyton floccosum, belonging to the dermatophytes group. Extraction products underwent polymerase chain reaction (PCR) fingerprinting with the appropriate primers to point out any disagreement in the genomic profiles. After having determined that the genomic profiles obtained from the DNA extracted from the same strain with the two methods correspond perfectly, the authors concluded that the extraction method with magnetic beads from fungal cells is simpler and quicker than with P/C extraction, greatly facilitating the obtainment of fungal DNA.
ISSN:0933-7407
1439-0507
DOI:10.1111/j.1439-0507.2004.01030.x