Different P105 Promoter Activities among Natural Variants of Human Papillomavirus Type 18

Association between non-European variants of human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 and cervical lesions has been suggested. To compare the P105 promoter activity among 6 HPV-18 variants, their complete long control regions (LCRs), as well as that of HeLa cells, were cloned upstream...

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Bibliographic Details
Published in:The Journal of infectious diseases 2005-03, Vol.191 (5), p.739-742
Main Authors: Sichero, Laura, Franco, Eduardo Luis, Villa, Luisa Lina
Format: Article
Language:English
Subjects:
Base Sequence
Binding sites
Biological and medical sciences
Cell lines
Cervical cancer
Fundamental and applied biological sciences. Psychology
Genes, Viral - physiology
Genetic Variation
HeLa Cells
Human papillomavirus 16
Human papillomavirus 18
Humans
Infectious diseases
Lesions
Medical sciences
Microbiology
Miscellaneous
Neoplasia
Nucleotides
Papillomaviridae - classification
Papillomaviridae - genetics
Phylogenetics
Phylogeny
Plasmids
Promoter Regions, Genetic - genetics
Species Specificity
Virology
Viruses
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Summary:Association between non-European variants of human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 and cervical lesions has been suggested. To compare the P105 promoter activity among 6 HPV-18 variants, their complete long control regions (LCRs), as well as that of HeLa cells, were cloned upstream of the luciferase gene and transiently transfected in C33 cells. Whereas the B18-2 European variant showed the lowest promoter activity, the Asian-Amerindian variant, B18-3, had the highest activity. All variants tested were more active than the P97 HPV-16 prototype promoter. Differences in the LCR that impact P105 promoter activity could be responsible for the observed variability in oncogenic potential
ISSN:0022-1899
1537-6613
DOI:10.1086/427825