Screening for Bacillus subtilis mutants deficient in pressure induced spore germination: identification of ykvU as a novel germination gene

Exposure to high pressure induces germination in spores of Bacillus subtilis. To investigate the mechanisms of this process and to compare the pressure and nutrient induced germination pathways, a random transposon knock-out library of B. subtilis was constructed and screened for clones with a compr...

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Bibliographic Details
Published in:FEMS microbiology letters 2005-02, Vol.243 (2), p.385-391
Main Authors: Aertsen, Abram, Opstal, Isabelle Van, Vanmuysen, Suzy C., Wuytack, Elke Y., Michiels, Chris W.
Format: Article
Language:English
Subjects:
Alanine
Amines - pharmacology
Bacillus subtilis
Bacillus subtilis - genetics
Bacillus subtilis - physiology
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Calcium
Calcium ions
Culture Media - chemistry
Dodecylamine
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genetics
Germination
High pressure
Hydrostatic Pressure
L-Alanine
Microbiology
Mutation
Nutrients
Pressure
Spore germination
Spores
Spores, Bacterial - physiology
Transposons
ykvU
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Summary:Exposure to high pressure induces germination in spores of Bacillus subtilis. To investigate the mechanisms of this process and to compare the pressure and nutrient induced germination pathways, a random transposon knock-out library of B. subtilis was constructed and screened for clones with a compromised pressure induced germination at 100 MPa. Two mutants were isolated and their transposon insertion was mapped to gerAC and ykvU respectively. While GerAC is required for production of the l-alanine receptor which has been implicated in pressure-induced germination before, YkvU is shown here to be a novel germination determinant in B. subtilis, affecting germination by high (100 MPa) and very high (600 MPa) pressure, by nutrients and by dodecylamine, but not by Ca 2+-dipicolinic acid.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2004.12.029