Selective separation of human serum albumin with copper(II) chelated poly(hydroxyethyl methacrylate) based nanoparticles

• Published in: International journal of biological macromolecules 2009-08, Vol.45 (2), p.188-193
• Main Authors: Karakoc, Veyis, Yılmaz, Erkut, Türkmen, Deniz, Öztürk, Nevra, Akgöl, Sinan, Denizli, Adil
• Format: Article
• Language: English
• Subjects: Adsorption; Albumin purification; Chelating Agents - chemical synthesis; Chelating Agents - chemistry; Copper - chemistry; Humans; Metal-chelates; Nanoparticles; Nanoparticles - chemistry; Polyhydroxyethyl Methacrylate - chemical synthesis; Polyhydroxyethyl Methacrylate - chemistry; Serum Albumin - chemistry; Serum Albumin - isolation & purification; Silanes - chemistry; Spectroscopy, Fourier Transform Infrared; Time Factors
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2009.04.023

Poly(hydroxyethyl methacrylate) (PHEMA) nanoparticles with an average size of 300 nm in diameter and with a polydispersity index of 1.156 were produced by surfactant free emulsion polymerization. Specific surface area of the PHEMA nanoparticles was found to be 996 m 2/g. Metal-chelating ligand 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) was covalently attached to the PHEMA nanoparticles. IMEO content was 0.97 mmol IEMO/g. The morphology and properties of these nanoparticles were characterized with scanning electron microscopy, Fourier transform infrared spectroscopy and atomic force microscopy. The Cu 2+-chelated PHEMA–IMEO nanoparticles were used in the adsorption-elution studies of human serum albumin (HSA) in a batch system. Maximum HSA adsorption amount of the Cu 2+ chelated nanoparticles was 680 mg HSA/g. The PHEMA–IMEO–Cu 2+ nanoparticles exhibited a quite high adsorption capacity and fast adsorption rate due to their high specific surface area and the absence of internal diffusion resistance.