Detection of the saa gene in verotoxin-producing Escherichia coli from ruminants

Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. A total of 163 verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrheic and healthy cattle, sheep, and goats were analyzed for the presence of the saa gene by polymerase chain re...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2005-01, Vol.17 (1), p.65-67
Main Authors: Orden, JA, Cortes, C, Ruiz-Santa-quiteria, JA, Martinez, S, de la Fuente, R
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - genetics
Animals
Cattle
Cattle Diseases - microbiology
Diarrhea - microbiology
Diarrhea - veterinary
Escherichia coli - genetics
Escherichia coli - pathogenicity
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Escherichia coli Proteins - genetics
Feces - microbiology
Goat Diseases - microbiology
Goats
Hemolysin Proteins - genetics
Sheep
Sheep Diseases - microbiology
Shiga Toxins - biosynthesis
Species Specificity
Virulence - genetics
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Summary:Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. A total of 163 verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrheic and healthy cattle, sheep, and goats were analyzed for the presence of the saa gene by polymerase chain reaction. Seventeen (45.9%) and 5 (29.4%) of the VTEC isolated from healthy cattle and diarrheic calves, respectively, had the saa gene. None of the saa-positive strains carried the eae gene, but 20 of the 22 saa positive were ehxA positive. In contrast with cattle VTEC, none of the VTEC isolated from small ruminants were saa positive. These results show that the saa gene is commonly associated with bovine eae-negative VTEC strains but not with ovine or caprine VTEC strains.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870501700113