Mineral nutrition and adventitious rooting in microcuttings of Eucalyptus globulus

We characterized the adventitious rooting response of Eucalyptus globulus Labill. to various concentrations of calcium, nitrogen, phosphorus, iron, manganese, zinc, boron and copper. The parameters analyzed were percent rooting, root number, root length and mean rooting time. Root number and root le...

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Bibliographic Details
Published in:Tree physiology 2005-04, Vol.25 (4), p.487-494
Main Authors: Schwambach, J, Fadanelli, C, Fett-Neto, A.G
Format: Article
Language:English
Subjects:
adventitious roots
culture media
Culture Media - chemistry
Elements
Eucalyptus - growth & development
Eucalyptus globulus
forest trees
in vitro culture
in vitro regeneration
methodology
micropropagation
minerals
nutrient requirements
plant cuttings
Plant Roots - growth & development
rooting
Tissue Culture Techniques
tree nutrition
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Summary:We characterized the adventitious rooting response of Eucalyptus globulus Labill. to various concentrations of calcium, nitrogen, phosphorus, iron, manganese, zinc, boron and copper. The parameters analyzed were percent rooting, root number, root length and mean rooting time. Root number and root length were significantly affected by mineral nutrition, whereas mean rooting time and rooting percentage seemed to be closely related to auxin availability. Root number was affected by calcium, nitrogen source and zinc, whereas root length was influenced by concentrations of phosphorus, iron and manganese, and by nitrogen source. Based on these results, we evaluated various combinations of several concentrations of these minerals in each rooting phase. Cuttings that were rooted in an optimized mineral nutrient medium and acclimatized to ex-vitro conditions for two months showed significantly higher survival after transplanting and drought stress than cuttings rooted in basal medium and treated in the same way.
ISSN:0829-318X
1758-4469
DOI:10.1093/treephys/25.4.487