Sulfated glycosaminoglycans in the extracellular matrix of muscle tissue in Atlantic cod ( Gadus morhua) and Spotted wolffish ( Anarhichas minor)

Two species of commercially important cold water fish were investigated for content of sulfated glycosaminoglycans (GAGs) in muscle tissue by use of in vivo 35 S-sulfate labeling combined with different digestions (papain, chondroitinase ABC, keratanase and nitrous acid treatment), DEAE chromatograp...

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Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2005-03, Vol.140 (3), p.349-357
Main Authors: Tingbø, Monica G., Kolset, Svein O., Ofstad, Ragni, Enersen, Grethe, Hannesson, Kirsten O.
Format: Article
Language:English
Subjects:
35S-labeling
Anarhichas minor
Animals
Chondroitin Sulfates - analysis
Dermatan Sulfate - analysis
Extracellular matrix
Extracellular Matrix - chemistry
Fish
Gadus morhua
Gadus morhua - metabolism
Glycosaminoglycans
Heparitin Sulfate - analysis
Histology
isotope labeling
Marine
muscle tissues
Muscles - chemistry
Perciformes - metabolism
sulfates
Sulfation
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Summary:Two species of commercially important cold water fish were investigated for content of sulfated glycosaminoglycans (GAGs) in muscle tissue by use of in vivo 35 S-sulfate labeling combined with different digestions (papain, chondroitinase ABC, keratanase and nitrous acid treatment), DEAE chromatography, SDS–PAGE and histology techniques. The species investigated in this study have different gaping properties. The non-gaping species, spotted wolffish ( Anarhichas minor), contained 3–4 times more 35 S-sulfated anionic components than the gaping species, Atlantic cod ( Gadus morhua). The higher level of sulfation in wolffish was supported by light microscopy studies using Alcian blue staining with different concentrations of MgCl 2 as critical electrolyte. Furthermore, the muscular connective tissue in the non-gaping species was dominated by chondroitin sulfate (CS)/dermatan sulfate (DS), whereas the gaping species was more dominated by heparan sulfate (HS). Moreover, structural differences were observed in the junctions between the myofibers, which were more pronounced in the wolffish. The histological studies revealed that the basement membrane area was rich in acidic mucopolysaccharides in both species.
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpc.2004.09.032