5-Hydroxytryptamine synthesis in HL-1 cells and neonatal rat cardiocytes

Some reports showed that serotonergic system might have existed and that 5-hydroxytryptamine (5-HT) was detected in the hamster heart. The source of 5-HT in the heart, however, remains to be fully elucidated. So the present study was designed to define serotonergic system and to clarify which cell c...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-03, Vol.328 (2), p.522-525
Main Authors: Ikeda, Keiichi, Tojo, Katsuyoshi, Otsubo, Chikara, Udagawa, Takashi, Kumazawa, Kensuke, Ishikawa, Masahiro, Tokudome, Goro, Hosoya, Tatsuo, Tajima, Naoko, Claycomb, William C., Nakao, Kazuwa, Kawamura, Masahiro
Format: Article
Language:English
Subjects:
5-Hydroxytryptamine
Animals
Animals, Newborn
Cell Line
Cells, Cultured
HL-1 cardiomyocytes
HLPC
Myocytes, Cardiac - metabolism
Neonatal rat cardiac myocytes
Neonatal rat cardiac non-myocytes
Rats
Rats, Wistar
Serotonin - biosynthesis
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Summary:Some reports showed that serotonergic system might have existed and that 5-hydroxytryptamine (5-HT) was detected in the hamster heart. The source of 5-HT in the heart, however, remains to be fully elucidated. So the present study was designed to define serotonergic system and to clarify which cell could produce 5-HT in the heart. As a result, 5-HT was detected in homogenates of HL-1 cardiomyocytes by high performance liquid chromatography with fluorescence detection, but not in those of neonatal rat non-cardiomyocytes (NMCs). And TPH and AADC mRNAs were expressed in HL-1 cardiomyocytes and neonatal rat cardiomyocytes (MCs), not in NMCs. mRNAs of 5-HT 2A receptor were detected in both MCs and NMCs, and those of 5-HT 2B receptor in NMCs. These findings definitively demonstrate that 5-HT is secreted from the myocytes of the heart and strongly implied that 5-HT might play a certain role in cardiac physiology.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.01.018