Use of the placental perfusion model to evaluate transplacental passage of Trypanosoma cruzi

To determine whether Trypanosoma cruzi could be identified in placental cells in vitro and in placental tissue using a human ex vivo perfusion model. A placental cell line was incubated with trypomastigotes for progressive time periods. Trypomastigotes were also infused as a bolus into the maternal...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2005-02, Vol.192 (2), p.586-591
Main Authors: Shippey, Stuart H., Zahn, Christopher M., Cisar, Margaret M., Wu, T. John, Satin, Andrew J.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cells, Cultured
Chagas Disease - congenital
Chagas Disease - transmission
Congenital Chagas' disease
Diseases of mother, fetus and pregnancy
DNA, Protozoan - analysis
Female
Gynecology. Andrology. Obstetrics
Human protozoal diseases
Humans
Infectious Disease Transmission, Vertical
Infectious diseases
Medical sciences
Parasitic diseases
Perfusion
Placenta - parasitology
Placental perfusion model
Pregnancy
Pregnancy. Fetus. Placenta
Protozoal diseases
Transplacental infection
Trophoblasts - parasitology
Trypanosoma cruzi
Trypanosoma cruzi - isolation & purification
Trypanosomiasis
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Summary:To determine whether Trypanosoma cruzi could be identified in placental cells in vitro and in placental tissue using a human ex vivo perfusion model. A placental cell line was incubated with trypomastigotes for progressive time periods. Trypomastigotes were also infused as a bolus into the maternal circulation of a placental perfusion model. Maternal and fetal perfusates, and placental tissue, were analyzed for parasitic DNA using polymerase chain reaction; perfused specimens were also examined histologically. Intracytoplasmic amastigotes were identified in trophoblast of the incubated cell line by 24–48 hours. Following placental perfusion, T cruzi DNA was identified in all postinoculation maternal perfusate samples and postinoculation placental tissue specimens; preinoculation controls were negative. This is the first description of the use of the human placental perfusion model to study congenital Chagas' disease, including the presence and time course of early parasitic invasion of the placenta.
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2004.07.079