Does HPV play a role in the etiopathogenesis of ameloblastoma? An immunohistochemical, in situ hybridization and polymerase chain reaction study of 18 cases using laser capture microdissection

Ameloblastomas are epithelial tumors of odontogenic origin, biologically characterized by local recurrence. Among different etiologic factors, HPV infection has been recently postulated to be somehow involved in ameloblastoma etiopathogenesis. To address this issue, we studied 18 ameloblastomas by m...

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Bibliographic Details
Published in:Modern pathology 2005-02, Vol.18 (2), p.283-289
Main Authors: Migaldi, Mario, Pecorari, Monica, Rossi, Giulio, Maiorana, Antonio, Bettelli, Stefania, Tamassia, Maria G, De Gaetani, Carmela, Leocata, Pietro, Portolani, Marinella
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
ameloblastoma
Ameloblastoma - etiology
Ameloblastoma - pathology
Antibodies
Female
HPV
Human papillomavirus
Humans
Hybridization
Immunohistochemistry
In Situ Hybridization
Infections
Jaw Neoplasms - etiology
Jaw Neoplasms - pathology
Laboratory Medicine
laser microdissection
Lasers
Male
Medicine
Medicine & Public Health
Microdissection - instrumentation
Microdissection - methods
Middle Aged
Oncogene Proteins, Viral - analysis
Oncogene Proteins, Viral - genetics
original-article
Papillomaviridae - genetics
Papillomaviridae - physiology
Papillomavirus Infections - complications
Papillomavirus Infections - virology
Pathogenesis
Pathology
PCR
Polymerase Chain Reaction
Tumors
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Summary:Ameloblastomas are epithelial tumors of odontogenic origin, biologically characterized by local recurrence. Among different etiologic factors, HPV infection has been recently postulated to be somehow involved in ameloblastoma etiopathogenesis. To address this issue, we studied 18 ameloblastomas by means of immunohistochemistry, in situ hybridization (conventional and amplified), polymerase chain reaction and nested-polymerase chain reaction analyses using laser capture microdissection in order to detect the occurrence of HPV in this setting. No evidence of HPV infection was detected by morphological examination, immunohistochemistry, in situ hybridization and conventional polymerase chain reaction, while nested-polymerase chain reaction showed a weak positive band in two cases. However, the subsequent restriction enzyme analysis carried out from the nested-polymerase chain reaction amplification products of these two samples excluded the presence of HPV subtypes 16, 18, 31, 33, 35, 52, and 58. The search for HPV 6 and 11 in the same specimens was also negative. In conclusion, our data do not support an etiopathogenetic evidence for HPV in ameloblastoma.
ISSN:0893-3952
1530-0285
DOI:10.1038/modpathol.3800241