Virulence attenuation in Salmonella enterica rcsC mutants with constitutive activation of the Rcs system

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, 41080 Sevilla, Spain Correspondence Francisco Ramos-Morales framos{at}us.es Mutations in rcsC that result in constitutive colanic acid capsule synthesis were obtained in Salmonella enterica serovar Typhimurium. Mo...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2005-02, Vol.151 (2), p.579-588
Main Authors: Garcia-Calderon, Clara B, Garcia-Quintanilla, Meritxell, Casadesus, Josep, Ramos-Morales, Francisco
Format: Article
Language:English
Subjects:
Animals
Bacterial Capsules - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Multienzyme Complexes - genetics
Multienzyme Complexes - metabolism
Mutation
Phosphoprotein Phosphatases - genetics
Phosphoprotein Phosphatases - metabolism
Polysaccharides - metabolism
Protein Kinases - genetics
Protein Kinases - metabolism
Salmonella Infections - microbiology
Salmonella typhimurium - genetics
Salmonella typhimurium - metabolism
Salmonella typhimurium - pathogenicity
Transcription Factors - genetics
Virulence
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Summary:Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, 41080 Sevilla, Spain Correspondence Francisco Ramos-Morales framos{at}us.es Mutations in rcsC that result in constitutive colanic acid capsule synthesis were obtained in Salmonella enterica serovar Typhimurium. Most rcsC alleles were dominant; however, recessive rcsC alleles were also found, in agreement with the postulated double role (positive and negative) of RcsC on the activation of the RcsB/C phosphorelay system. Salmonella rcsC mutants with constitutive activation of the Rcs system are severely attenuated for virulence in BALB/c mice and their degree of attenuation correlates with the level of Rcs activation. Partial relief of attenuation by a gmm mutation indicates that capsule overproduction is one of the factors leading to avirulence in constitutively activated rcsC mutants. Abbreviations: CI, competitive index; Km, kanamycin
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.27520-0