High-performance liquid chromatographic determination of phenolic compounds in rice

A method has been developed for the determination of 6′- O-feruloylsucrose, 6′- O-sinapoylsucrose, ferulic acid, sinapinic acid, p-coumaric acid, chlorogenic (3-caffeoylquinic) acid, caffeic acid, protocatechuic acid, hydroxybenzoic acid, vanillic acid, and syringic acid in rice. The rice samples we...

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Bibliographic Details
Published in:Journal of Chromatography A 2005-01, Vol.1063 (1), p.121-128
Main Authors: Tian, Su, Nakamura, Kozo, Cui, Tong, Kayahara, Hiroshi
Format: Article
Language:English
Subjects:
6′- O-Feruloylsucrose
6′- O-Sinapoylsucrose
Agronomy. Soil science and plant productions
Biological and medical sciences
Cereal and baking product industries
Chemical constitution
Chromatography, High Pressure Liquid - methods
Economic plant physiology
Food industries
Fundamental and applied biological sciences. Psychology
Germinated brown rice
Oryza - chemistry
Phenolic compounds
Phenols - analysis
Rice
Sensitivity and Specificity
Solid-phase extraction
Spectrometry, Mass, Electrospray Ionization
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Summary:A method has been developed for the determination of 6′- O-feruloylsucrose, 6′- O-sinapoylsucrose, ferulic acid, sinapinic acid, p-coumaric acid, chlorogenic (3-caffeoylquinic) acid, caffeic acid, protocatechuic acid, hydroxybenzoic acid, vanillic acid, and syringic acid in rice. The rice samples were extracted with 70% ethanol, filtered, and defatted. The defatted aqueous solution was subjected to solid-phase extraction using a C 18 silica gel cartridge; no analyte was lost in this procedure. The 70% acidic methanol elution was analyzed directly by HPLC and HPLC-ESI-MS. Phenolic compounds were separated with a C 18 reversed-phase column by gradient elution using 0.025% trifluoroacetic acid in purified water (A)—acetonitrile (B) (0 min, 5% B; 5 min, 9% B; 15 min, 9% B; 22 min, 11% B; and 38 min, 18% B) as the mobile phase at a flow rate of 0.8 ml/min. Detection limits ranged from 0.10 to 0.35 ng per injection (5 μl). Relative standard deviations of 0.22–3.95% and recoveries of 99–108% were obtained for simultaneous determination of these phenolic compounds. This method was applied to analysis of phenolic compounds in brown rice and germinated brown rice soaked in 32 °C water for varying durations.
ISSN:0021-9673
DOI:10.1016/j.chroma.2004.11.075