Two-state migration of DNA in a structured microchannel

DNA migration in topologically structured microchannels with periodic cavities is investigated experimentally and with Brownian dynamics simulations of a simple bead-spring model. The results are in very good agreement with one another. In particular, the experimentally observed migration order of l...

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Bibliographic Details
Published in:Physical review. E, Statistical, nonlinear, and soft matter physics Statistical, nonlinear, and soft matter physics, 2005-01, Vol.71 (1 Pt 1), p.011905-011905, Article 011905
Main Authors: Streek, Martin, Schmid, Friederike, Duong, Thanh Tu, Anselmetti, Dario, Ros, Alexandra
Format: Article
Language:English
Subjects:
Computer Simulation
Diffusion - radiation effects
DNA - chemistry
DNA - isolation & purification
DNA - radiation effects
Electrochemistry - methods
Electromagnetic Fields
Electrophoresis - methods
Microfluidics - methods
Models, Chemical
Motion
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Summary:DNA migration in topologically structured microchannels with periodic cavities is investigated experimentally and with Brownian dynamics simulations of a simple bead-spring model. The results are in very good agreement with one another. In particular, the experimentally observed migration order of lambda - and T2-DNA molecules is reproduced by the simulations. The simulation data indicate that the mobility may depend on the chain length in a nonmonotonic way at high electric fields. This is found to be the signature of a nonequilibrium bistability between two different migration states, a slow one and a fast one. The latter can also be observed experimentally under appropriate conditions.
ISSN:1539-3755
1550-2376
DOI:10.1103/PhysRevE.71.011905