Chitosan nano-/microfibrous double-layered membrane with rolled-up three-dimensional structures for chondrocyte cultivation

With an aim to mimic natural extracellular matrix, we fabricated the nano‐ and microfibrous matrix with chitosan by electrospinning nanofibers onto predefined microfibrous mesh for effective chondrocytes cultivation. Rolling the double‐layered nano‐/microfibrous membranes produced three‐dimensional...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2009-08, Vol.90A (2), p.595-602
Main Authors: Shim, In Kyong, Suh, Won Hee, Lee, Sang Young, Lee, Sang Hoon, Heo, Seong Joo, Lee, Myung Chul, Lee, Seung Jin
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - chemistry
biomimetic
Biomimetics
Cartilage - pathology
Cartilage - physiology
Cattle
Cell Culture Techniques - methods
Cell Proliferation
Cells, Cultured
chitosan
Chitosan - chemistry
chondrocyte
Chondrocytes - cytology
Chondrocytes - metabolism
Electrochemistry - methods
electrospinning
Microscopy, Electron, Scanning - methods
nanofiber
Nanotechnology - methods
Polymers - chemistry
Tissue Engineering - methods
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Summary:With an aim to mimic natural extracellular matrix, we fabricated the nano‐ and microfibrous matrix with chitosan by electrospinning nanofibers onto predefined microfibrous mesh for effective chondrocytes cultivation. Rolling the double‐layered nano‐/microfibrous membranes produced three‐dimensional (3‐D) scaffolds that exhibited the interconnected open pore structure in their scanning electron microscopy images. In vitro chondrocyte culture experiment showed that this nano‐/microfibrous 3‐D matrix provided a significantly greater microenvironment for chondrocytes to proliferate and produce glycosaminoglycan as compared with only microfibrous 3‐D matrix. This difference could be explained by the result on 2‐D membrane, where chitosan nanofibrous surface substantially facilitated the cellular attachment and proliferation, and efficiently prevented phenotypic changes of chondrocytes, when compared with chitosan microfibrous membrane and film. In this regard, the nano‐/microfibrous 3‐D matrix we fabricated in this study would possess a great potential as a system for effective chondrocyte cultivation and also for application to cartilage regeneration therapy. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.32109