Developmental expression of glucokinase in rat hypothalamus

Neurons in the hypothalamus sense changes in glucose concentration. Glucokinase (GK), a key enzyme for pancreatic (β)-cell glucose sensing, was found in both the embryonic and adult hypothalamus. GK activity accounted for approximately 20% of total hexokinase (HK) activity in both embryonic and adul...

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Bibliographic Details
Published in:Brain research. Developmental brain research 2005-02, Vol.154 (2), p.255-258
Main Authors: Sutherland, Vicki L., McReynolds, Matthew, Tompkins, Linda S., Brooks, Heddwen L., Lynch, Ronald M.
Format: Article
Language:English
Subjects:
Age Factors
Animals
Animals, Newborn
Blotting, Northern - methods
Blotting, Southern - methods
Development
DNA, Complementary - metabolism
Embryo, Mammalian
Gene Expression Regulation, Developmental - physiology
Glucokinase
Glucokinase - genetics
Glucokinase - metabolism
Glucose - analysis
Glucose sensing
Hexokinase - metabolism
Hypothalamus - cytology
Hypothalamus - embryology
Hypothalamus - enzymology
Hypothalamus - growth & development
In Vitro Techniques
Polymerase Chain Reaction - methods
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
RT–PCR
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Summary:Neurons in the hypothalamus sense changes in glucose concentration. Glucokinase (GK), a key enzyme for pancreatic (β)-cell glucose sensing, was found in both the embryonic and adult hypothalamus. GK activity accounted for approximately 20% of total hexokinase (HK) activity in both embryonic and adult hypothalamus with no activity measured in cortical samples, indicating that glucose sensing in the hypothalamus initiates early in development and precedes the maturation of glucose signaling in liver.
ISSN:0165-3806
DOI:10.1016/j.devbrainres.2004.11.001