Paratuberculosis and avian tuberculosis infections in one red deer farm studied by IS900 and IS901 RFLP analysis

• Published in: Veterinary microbiology 2005-02, Vol.105 (3-4), p.261-268
• Main Authors: Machackova-Kopecna, M, Bartos, M, Straka, M, Ludvik, V, Svastova, P, Alvarez, J, Lamka, J, Trcka, I, Treml, F, Parmova, I
• Format: Article
• Language: English
• Subjects: animal diseases; Animals; avian tuberculosis; case studies; Cervus elaphus; Czech Republic - epidemiology; Deer - microbiology; disease detection; disease diagnosis; disease outbreaks; epidemiology; farms; Female; game animals; histopathology; Ileum - microbiology; Ileum - pathology; Liver - microbiology; Liver - pathology; Lymph Nodes - microbiology; Lymph Nodes - pathology; Male; microbial detection; mixed infection; Mycobacterium avium; Mycobacterium avium - classification; Mycobacterium avium - genetics; Mycobacterium avium - isolation & purification; Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. paratuberculosis - classification; Mycobacterium avium subsp. paratuberculosis - genetics; Mycobacterium avium subsp. paratuberculosis - isolation & purification; paratuberculosis; Paratuberculosis - epidemiology; Paratuberculosis - microbiology; Paratuberculosis - pathology; parks; pathogen identification; Polymorphism, Restriction Fragment Length; restriction fragment length polymorphism; Spleen - microbiology; Spleen - pathology; Tuberculosis - epidemiology; Tuberculosis - microbiology; Tuberculosis - veterinary
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2004.11.008

As the attempt to eradicate paratuberculosis in one red deer (Cervus elaphus) farm failed, all 167 red deer of different age groups were slaughtered and examined by culture for mycobacteria, and the farm was closed down. Spleen and hepatic lymph nodes, mediastinal lymph node, ileocecal lymph node, and ileum were collected from each animal and examined (a total of 835 organs). Neither tuberculosis lesions nor pathognomic signs of paratuberculosis were detected. Among all microscopically negative for mycobacteria organs, Mycobacterium avium subsp. paratuberculosis alone was isolated from 165 organs, M. a. avium alone from 41 organs, and both pathogens from four organs. M. a. paratuberculosis alone was detected in 71 red deer, M. a. avium alone in 13 red deer and both pathogens in 18 red deer. Using standardised RFLP methods, three IS900 RFLP types B-C1, B-C16, and B-C32 were identified among 40 M. a. paratuberculosis isolates and four IS901 RFLP types N-B1, N-B3, N-B4, and P-B3 among 17 M. a. avium isolates.